TY - JOUR
T1 - Production of 3-hyroxypropionic acid from acrylic acid by newly isolated Rhodococcus erythropolis LG12
AU - Lee, Sang Hyun
AU - Park, Si Jae
AU - Park, Oh Jin
AU - Chao, Junhyeong
AU - Rhee, Joo Won
PY - 2009/5/28
Y1 - 2009/5/28
N2 - A novel microorganism, designated as LG12, was isolated from soil based on its ability to use acrylic acid as the sole carbon source. An electron microscopic analysis of its morphological characteristics and phylogenetic classification by 16S rRNA homology showed that the LG12 strain belongs to Rhodococcus erythropolis. R. erythropolis LG12 was able to metabolize a high concentration of acrylic acid (up to 40 g/l). In addition, R. erythropolis LG12 exhibited the highest acrylic acid-degrading activity among the tested microorganisms, including R. rhodochrous, R. equi, R. rubber, Candida rugosa, and Bacillus cereus. The effect of the culture conditions of R. erythropolis LG12 on the production of 3-hydroxypropionic acid (3HP) from acrylic acid was also examined. To enhance the production of 3HP, acrylic acid-assimilating activity was induced by adding 1 mM acrylic acid to the culture medium when the cell density reached an OD600 of 5. Further cultivation of R. erythropolis LG12 with 40 g/l of acrylic acid resulted in the production of 17.5 g/l of 3HP with a molar conversion yield of 44% and productivity of 0.22 g/l/h at 30°C after 72 h.
AB - A novel microorganism, designated as LG12, was isolated from soil based on its ability to use acrylic acid as the sole carbon source. An electron microscopic analysis of its morphological characteristics and phylogenetic classification by 16S rRNA homology showed that the LG12 strain belongs to Rhodococcus erythropolis. R. erythropolis LG12 was able to metabolize a high concentration of acrylic acid (up to 40 g/l). In addition, R. erythropolis LG12 exhibited the highest acrylic acid-degrading activity among the tested microorganisms, including R. rhodochrous, R. equi, R. rubber, Candida rugosa, and Bacillus cereus. The effect of the culture conditions of R. erythropolis LG12 on the production of 3-hydroxypropionic acid (3HP) from acrylic acid was also examined. To enhance the production of 3HP, acrylic acid-assimilating activity was induced by adding 1 mM acrylic acid to the culture medium when the cell density reached an OD600 of 5. Further cultivation of R. erythropolis LG12 with 40 g/l of acrylic acid resulted in the production of 17.5 g/l of 3HP with a molar conversion yield of 44% and productivity of 0.22 g/l/h at 30°C after 72 h.
KW - 3-hydroxypropionic acid
KW - Acrylic acid
KW - Resting cell reaction
KW - Rhodococcus erythropolis LG12
UR - http://www.scopus.com/inward/record.url?scp=67649598597&partnerID=8YFLogxK
U2 - 10.4014/jmb.0808.473
DO - 10.4014/jmb.0808.473
M3 - Article
C2 - 19494695
AN - SCOPUS:67649598597
SN - 1017-7825
VL - 19
SP - 474
EP - 481
JO - Journal of Microbiology and Biotechnology
JF - Journal of Microbiology and Biotechnology
IS - 5
ER -