Production of 10-hydroxystearic acid from oleic acid by whole cells of recombinant Escherichia coli containing oleate hydratase from Stenotrophomonas maltophilia

Young Chul Joo, Eun Sun Seo, Yeong Su Kim, Kyoung Rok Kim, Jin Byung Park, Deok Kun Oh

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

A putative fatty acid hydratase from Stenotrophomonas maltophilia was cloned and expressed in Escherichia coli. The recombinant enzyme showed the highest hydration activity for oleic acid among the fatty acids tested, indicating that the enzyme is an oleate hydratase. The optimal conditions for the production of 10-hydroxystearic acid from oleic acid using whole cells of recombinant E. coli containing the oleate hydratase were pH 6.5, 35°C, 0.05% (w/v) Tween 40, 10gl -1 cells, and 50gl -1 oleic acid. Under these conditions, whole recombinant cells produced 49gl -1 10-hydroxystearic acid for 4h, with a conversion yield of 98% (w/w), a volumetric productivity of 12.3gl -1h -1, and a specific productivity of 1.23gg-cells -1h -1, which were 18%, 2.5-, and 2.5-fold higher than those of whole wild-type S. maltophilia cells, respectively. This is the first report of 10-hydroxystearic acid production using recombinant cells and the concentration and productivity are the highest reported thus far among cells.

Original languageEnglish
Pages (from-to)17-23
Number of pages7
JournalJournal of Biotechnology
Volume158
Issue number1-2
DOIs
StatePublished - 31 Mar 2012

Keywords

  • 10-Hydroxystearic acid production
  • Oleate hydratase
  • Oleic acid
  • Recombinant Escherichia coli
  • Stenotrophomonas maltophilia

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