Probing protease activity by single-fluorescent-protein nanocapsules

Zhen Gu; Anuradha Biswas; Kye Il Joo; Biliang Hu; Pin Wang; Yi Tang

doi:10.1039/c0cc01439g

Probing protease activity by single-fluorescent-protein nanocapsules

Zhen Gu, Anuradha Biswas, Kye Il Joo, Biliang Hu, Pin Wang, Yi Tang

Department of Chemical Engineering & Materials Science

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

We describe a FRET-based protease detection strategy, using a single-fluorescent-protein nanogel as donor and a dark quencher as acceptor linked by a photolabile caged-peptide. This design enables probing of protease activity in a UV-responsive fashion.

Original language	English
Pages (from-to)	6467-6469
Number of pages	3
Journal	Chemical Communications
Volume	46
Issue number	35
DOIs	https://doi.org/10.1039/c0cc01439g
State	Published - 21 Sep 2010

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title = "Probing protease activity by single-fluorescent-protein nanocapsules",

abstract = "We describe a FRET-based protease detection strategy, using a single-fluorescent-protein nanogel as donor and a dark quencher as acceptor linked by a photolabile caged-peptide. This design enables probing of protease activity in a UV-responsive fashion.",

author = "Zhen Gu and Anuradha Biswas and Joo, {Kye Il} and Biliang Hu and Pin Wang and Yi Tang",

year = "2010",

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T1 - Probing protease activity by single-fluorescent-protein nanocapsules

AU - Gu, Zhen

AU - Biswas, Anuradha

AU - Joo, Kye Il

AU - Hu, Biliang

AU - Wang, Pin

AU - Tang, Yi

PY - 2010/9/21

Y1 - 2010/9/21

N2 - We describe a FRET-based protease detection strategy, using a single-fluorescent-protein nanogel as donor and a dark quencher as acceptor linked by a photolabile caged-peptide. This design enables probing of protease activity in a UV-responsive fashion.

AB - We describe a FRET-based protease detection strategy, using a single-fluorescent-protein nanogel as donor and a dark quencher as acceptor linked by a photolabile caged-peptide. This design enables probing of protease activity in a UV-responsive fashion.

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U2 - 10.1039/c0cc01439g

DO - 10.1039/c0cc01439g

M3 - Article

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AN - SCOPUS:77956023955

SN - 1359-7345

VL - 46

SP - 6467

EP - 6469

JO - Chemical Communications

JF - Chemical Communications

IS - 35

ER -

Probing protease activity by single-fluorescent-protein nanocapsules

Abstract

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