Background: The purpose of this study was to investigate the effects of tonsil-derived mesenchymal stem cells (MSCs) on osteoradionecrosis (ORN). Method: We generated a mandibular ORN rat model using a combination of 20-Gy single-dose irradiation and tooth extraction. Study groups were negative control (tooth extraction only), ORN group (irradiation, tooth extraction), Matrigel-1 group (Matrigel; BD Biosciences, San Jose, CA; irradiation, Matrigel application immediately after tooth extraction), tonsil-derived MSC-1 group (irradiation, tonsil-derived MSC application immediately after tooth extraction), Matrigel-4 group (irradiation, Matrigel application 4 weeks after tooth extraction), and tonsil-derived MSC-4 group (irradiation, tonsil-derived MSC application 4 weeks after tooth extraction). Result: Bone mineral density was significantly lower in the ORN group than in the negative control group. The tonsil-derived MSC-1 group showed significantly higher bone mineral density than did the ORN and tonsil-derived MSC-4 groups. Conclusion: A single 20-Gy dose of irradiation combined with tooth extraction successfully generated ORN in the rat model. The tonsil-derived MSCs can be effective for bone regeneration in ORN, particularly when applied immediately after dentoalveolar trauma or surgery.
Bibliographical noteFunding Information:
This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government, (NRF-2015R1D1A1A 02062345) and (NRF-2017M3A9E 8033206), Republic of Korea.
This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government, (NRF-2015R1D1A1A02062345) and, (NRF-2017M3 A9E8033206) Republic of Korea.
© 2017 Wiley Periodicals, Inc.
- animal model
- cell therapy
- mesenchymal stem cell