Preventive effects of tonsil-derived mesenchymal stem cells on osteoradionecrosis in a rat model

Hae Sang Park, Jihae Lee, Jin Woo Kim, Ha Young Kim, Soo Yeon Jung, Sung Min Lee, Chan Hum Park, Han Su Kim

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Background: The purpose of this study was to investigate the effects of tonsil-derived mesenchymal stem cells (MSCs) on osteoradionecrosis (ORN). Method: We generated a mandibular ORN rat model using a combination of 20-Gy single-dose irradiation and tooth extraction. Study groups were negative control (tooth extraction only), ORN group (irradiation, tooth extraction), Matrigel-1 group (Matrigel; BD Biosciences, San Jose, CA; irradiation, Matrigel application immediately after tooth extraction), tonsil-derived MSC-1 group (irradiation, tonsil-derived MSC application immediately after tooth extraction), Matrigel-4 group (irradiation, Matrigel application 4 weeks after tooth extraction), and tonsil-derived MSC-4 group (irradiation, tonsil-derived MSC application 4 weeks after tooth extraction). Result: Bone mineral density was significantly lower in the ORN group than in the negative control group. The tonsil-derived MSC-1 group showed significantly higher bone mineral density than did the ORN and tonsil-derived MSC-4 groups. Conclusion: A single 20-Gy dose of irradiation combined with tooth extraction successfully generated ORN in the rat model. The tonsil-derived MSCs can be effective for bone regeneration in ORN, particularly when applied immediately after dentoalveolar trauma or surgery.

Original languageEnglish
Pages (from-to)526-535
Number of pages10
JournalHead and Neck
Volume40
Issue number3
DOIs
StatePublished - Mar 2018

Keywords

  • animal model
  • cell therapy
  • mesenchymal stem cell
  • osteoradionecrosis
  • tonsils

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