Pretreatment of kenaf (Hibiscus cannabinus L.) biomass feedstock for polyhydroxybutyrate (PHB) production and characterization

Rijuta Ganesh Saratale, Ganesh Dattatraya Saratale, Si Kyung Cho, Dong Su Kim, Gajanan S. Ghodake, Avinash Kadam, Gopalakrishanan Kumar, Ram Naresh Bharagava, Rajesh Banu, Han Seung Shin

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Kenaf biomass (KB) was employed as feedstock for the synthesis of polyhydroxybutyrate (PHB) using Ralstonia eutropha to replace conventional petroleum-derived polymers. Various pretreatments followed by enzymatic saccharification were applied to release monomeric sugars from KB for PHB production. The effects of increasing concentration of Na2CO3 + Na2SO3 (NaC + NaS) pretreated KB hydrolysates (20–40 g/L) on PHB production were investigated. NaC + NaS pretreated KB hydrolysates (30 g/L) exhibited maximum 70.0% PHA accumulation, with PHB titers of 10.10 g/L and PHB yields of about 0.488 g/g of reducing sugar produced within 36 h of fermentation. PHA accumulation, PHB yield and R. eutropha growth performance using KB hydrolysates were found to be comparable with those of synthetic sugar mixture. Characterization of the produced PHB in terms of crystalline structure, and thermal properties was done using various analytical techniques and results coincide with standard PHB. Thus, green liquor pretreated KB hydrolysates deliver a promising and economically feasible carbon substrate for PHB production.

Original languageEnglish
Pages (from-to)75-80
Number of pages6
JournalBioresource Technology
StatePublished - Jun 2019

Bibliographical note

Funding Information:
This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through High Value-added Food Technology Development Program funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (118056-2) and funded by Korea Environmental Industry & Technology Institute (A117-00197-0703-0).

Publisher Copyright:
© 2019


  • Enzymatic hydrolysis
  • Green liquor pretreatment
  • Kenaf biomass
  • Poly(3-hydroxybutyrate)
  • Ralstonia eutropha ATCC 17699


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