Pretreatment of kenaf (Hibiscus cannabinus L.) biomass feedstock for polyhydroxybutyrate (PHB) production and characterization

Rijuta Ganesh Saratale; Ganesh Dattatraya Saratale; Si Kyung Cho; Dong Su Kim; Gajanan S. Ghodake; Avinash Kadam; Gopalakrishanan Kumar; Ram Naresh Bharagava; Rajesh Banu; Han Seung Shin

doi:10.1016/j.biortech.2019.02.083

Pretreatment of kenaf (Hibiscus cannabinus L.) biomass feedstock for polyhydroxybutyrate (PHB) production and characterization

Rijuta Ganesh Saratale, Ganesh Dattatraya Saratale, Si Kyung Cho, Dong Su Kim, Gajanan S. Ghodake, Avinash Kadam, Gopalakrishanan Kumar, Ram Naresh Bharagava, Rajesh Banu, Han Seung Shin

Department of Environmental Science & Engineering

Research output: Contribution to journal › Article › peer-review

94 Scopus citations

Abstract

Kenaf biomass (KB) was employed as feedstock for the synthesis of polyhydroxybutyrate (PHB) using Ralstonia eutropha to replace conventional petroleum-derived polymers. Various pretreatments followed by enzymatic saccharification were applied to release monomeric sugars from KB for PHB production. The effects of increasing concentration of Na₂CO₃ + Na₂SO₃ (NaC + NaS) pretreated KB hydrolysates (20–40 g/L) on PHB production were investigated. NaC + NaS pretreated KB hydrolysates (30 g/L) exhibited maximum 70.0% PHA accumulation, with PHB titers of 10.10 g/L and PHB yields of about 0.488 g/g of reducing sugar produced within 36 h of fermentation. PHA accumulation, PHB yield and R. eutropha growth performance using KB hydrolysates were found to be comparable with those of synthetic sugar mixture. Characterization of the produced PHB in terms of crystalline structure, and thermal properties was done using various analytical techniques and results coincide with standard PHB. Thus, green liquor pretreated KB hydrolysates deliver a promising and economically feasible carbon substrate for PHB production.

Original language	English
Pages (from-to)	75-80
Number of pages	6
Journal	Bioresource Technology
Volume	282
DOIs	https://doi.org/10.1016/j.biortech.2019.02.083
State	Published - Jun 2019

Bibliographical note

Funding Information:
This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through High Value-added Food Technology Development Program funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (118056-2) and funded by Korea Environmental Industry & Technology Institute (A117-00197-0703-0).

Publisher Copyright:
© 2019

Keywords

Enzymatic hydrolysis
Green liquor pretreatment
Kenaf biomass
Poly(3-hydroxybutyrate)
Ralstonia eutropha ATCC 17699

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2019.02.083

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@article{91fb77f028d54e30b74f5cfd001293ad,

title = "Pretreatment of kenaf (Hibiscus cannabinus L.) biomass feedstock for polyhydroxybutyrate (PHB) production and characterization",

abstract = "Kenaf biomass (KB) was employed as feedstock for the synthesis of polyhydroxybutyrate (PHB) using Ralstonia eutropha to replace conventional petroleum-derived polymers. Various pretreatments followed by enzymatic saccharification were applied to release monomeric sugars from KB for PHB production. The effects of increasing concentration of Na2CO3 + Na2SO3 (NaC + NaS) pretreated KB hydrolysates (20–40 g/L) on PHB production were investigated. NaC + NaS pretreated KB hydrolysates (30 g/L) exhibited maximum 70.0% PHA accumulation, with PHB titers of 10.10 g/L and PHB yields of about 0.488 g/g of reducing sugar produced within 36 h of fermentation. PHA accumulation, PHB yield and R. eutropha growth performance using KB hydrolysates were found to be comparable with those of synthetic sugar mixture. Characterization of the produced PHB in terms of crystalline structure, and thermal properties was done using various analytical techniques and results coincide with standard PHB. Thus, green liquor pretreated KB hydrolysates deliver a promising and economically feasible carbon substrate for PHB production.",

keywords = "Enzymatic hydrolysis, Green liquor pretreatment, Kenaf biomass, Poly(3-hydroxybutyrate), Ralstonia eutropha ATCC 17699",

author = "Saratale, {Rijuta Ganesh} and Saratale, {Ganesh Dattatraya} and Cho, {Si Kyung} and Kim, {Dong Su} and Ghodake, {Gajanan S.} and Avinash Kadam and Gopalakrishanan Kumar and Bharagava, {Ram Naresh} and Rajesh Banu and Shin, {Han Seung}",

note = "Funding Information: This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through High Value-added Food Technology Development Program funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (118056-2) and funded by Korea Environmental Industry & Technology Institute (A117-00197-0703-0). Publisher Copyright: {\textcopyright} 2019",

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doi = "10.1016/j.biortech.2019.02.083",

language = "English",

volume = "282",

pages = "75--80",

journal = "Bioresource Technology",

issn = "0960-8524",

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AU - Saratale, Rijuta Ganesh

AU - Saratale, Ganesh Dattatraya

AU - Cho, Si Kyung

AU - Kim, Dong Su

AU - Ghodake, Gajanan S.

AU - Kadam, Avinash

AU - Kumar, Gopalakrishanan

AU - Bharagava, Ram Naresh

AU - Banu, Rajesh

AU - Shin, Han Seung

N1 - Funding Information: This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through High Value-added Food Technology Development Program funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (118056-2) and funded by Korea Environmental Industry & Technology Institute (A117-00197-0703-0). Publisher Copyright: © 2019

PY - 2019/6

Y1 - 2019/6

N2 - Kenaf biomass (KB) was employed as feedstock for the synthesis of polyhydroxybutyrate (PHB) using Ralstonia eutropha to replace conventional petroleum-derived polymers. Various pretreatments followed by enzymatic saccharification were applied to release monomeric sugars from KB for PHB production. The effects of increasing concentration of Na2CO3 + Na2SO3 (NaC + NaS) pretreated KB hydrolysates (20–40 g/L) on PHB production were investigated. NaC + NaS pretreated KB hydrolysates (30 g/L) exhibited maximum 70.0% PHA accumulation, with PHB titers of 10.10 g/L and PHB yields of about 0.488 g/g of reducing sugar produced within 36 h of fermentation. PHA accumulation, PHB yield and R. eutropha growth performance using KB hydrolysates were found to be comparable with those of synthetic sugar mixture. Characterization of the produced PHB in terms of crystalline structure, and thermal properties was done using various analytical techniques and results coincide with standard PHB. Thus, green liquor pretreated KB hydrolysates deliver a promising and economically feasible carbon substrate for PHB production.

AB - Kenaf biomass (KB) was employed as feedstock for the synthesis of polyhydroxybutyrate (PHB) using Ralstonia eutropha to replace conventional petroleum-derived polymers. Various pretreatments followed by enzymatic saccharification were applied to release monomeric sugars from KB for PHB production. The effects of increasing concentration of Na2CO3 + Na2SO3 (NaC + NaS) pretreated KB hydrolysates (20–40 g/L) on PHB production were investigated. NaC + NaS pretreated KB hydrolysates (30 g/L) exhibited maximum 70.0% PHA accumulation, with PHB titers of 10.10 g/L and PHB yields of about 0.488 g/g of reducing sugar produced within 36 h of fermentation. PHA accumulation, PHB yield and R. eutropha growth performance using KB hydrolysates were found to be comparable with those of synthetic sugar mixture. Characterization of the produced PHB in terms of crystalline structure, and thermal properties was done using various analytical techniques and results coincide with standard PHB. Thus, green liquor pretreated KB hydrolysates deliver a promising and economically feasible carbon substrate for PHB production.

KW - Enzymatic hydrolysis

KW - Green liquor pretreatment

KW - Kenaf biomass

KW - Poly(3-hydroxybutyrate)

KW - Ralstonia eutropha ATCC 17699

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AN - SCOPUS:85062405303

SN - 0960-8524

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JO - Bioresource Technology

JF - Bioresource Technology

ER -

Pretreatment of kenaf (Hibiscus cannabinus L.) biomass feedstock for polyhydroxybutyrate (PHB) production and characterization

Abstract

Bibliographical note

Keywords

UN SDGs

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