Presence of human mycoplasma DNA in gastric tissue samples from Korean chronic gastritis patients

Hyuk Joon Kwon; Jeong Ok Kang; Sun Hee Cho; Hee Bum Kang; Kyung Ah Kang; Jeong Ki Kim; Yoon Suk Kang; Byung Cheol Song; Hyun Wook Kang; Mi Ja Shim; Hee Sun Kim; Young Bae Kim; Ki Baeg Hahm; Bum Joon Kim; Myeong Cherl Kook; Myung Hee Chung; Jin Won Hyun

doi:10.1111/j.1349-7006.2004.tb03208.x

Presence of human mycoplasma DNA in gastric tissue samples from Korean chronic gastritis patients

Hyuk Joon Kwon, Jeong Ok Kang, Sun Hee Cho, Hee Bum Kang, Kyung Ah Kang, Jeong Ki Kim, Yoon Suk Kang, Byung Cheol Song, Hyun Wook Kang, Mi Ja Shim, Hee Sun Kim, Young Bae Kim, Ki Baeg Hahm, Bum Joon Kim, Myeong Cherl Kook, Myung Hee Chung, Jin Won Hyun

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

We aimed to determine whether mycoplasmas are present in Korean chronic gastritis, and to understand their roles in gastric cancer tumorigenesis, because mycoplasmas resemble Helicobacter pylori in terms of ammonia production and induction of inflammatory cytokines in immune and non-immune cells. The presence and identity of mycoplasmas were assessed by semi-nested PCR and sequencing, and the results were compared with pathologic data. Fifty-six samples collected from Korean chronic gastritis patients were used for this study. Twenty-three (41.1%) were positive for mycoplasmas. Eighteen sequenced samples contained a single human mycoplasma or two mycoplasmas, which were identified as Mycoplasma faucium (13/18), M. fermentans (3/18), M. orale (1/18), M. salivarium (2/18), and M. spermatophilum (1/ 18). Mycoplasma-infected chronic gastritis samples showed significantly more severe neutrophil infiltration than non-infected samples (P=0.0135). Mycoplasma profiles in the oral cavity (M. salivarium is major) and stomach were different, and the presence of significant proinflammatory responses in mycoplasma-positive patients suggests that the mycoplasmas are not simply contaminants. Further studies are required to understand whether mycoplasmas play a role in gastric tumorigenesis.

Original language	English
Pages (from-to)	311-315
Number of pages	5
Journal	Cancer Science
Volume	95
Issue number	4
DOIs	https://doi.org/10.1111/j.1349-7006.2004.tb03208.x
State	Published - Apr 2004

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Kwon, H. J., Kang, J. O., Cho, S. H., Kang, H. B., Kang, K. A., Kim, J. K., Kang, Y. S., Song, B. C., Kang, H. W., Shim, M. J., Kim, H. S., Kim, Y. B., Hahm, K. B., Kim, B. J., Kook, M. C., Chung, M. H., & Hyun, J. W. (2004). Presence of human mycoplasma DNA in gastric tissue samples from Korean chronic gastritis patients. Cancer Science, 95(4), 311-315. https://doi.org/10.1111/j.1349-7006.2004.tb03208.x

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abstract = "We aimed to determine whether mycoplasmas are present in Korean chronic gastritis, and to understand their roles in gastric cancer tumorigenesis, because mycoplasmas resemble Helicobacter pylori in terms of ammonia production and induction of inflammatory cytokines in immune and non-immune cells. The presence and identity of mycoplasmas were assessed by semi-nested PCR and sequencing, and the results were compared with pathologic data. Fifty-six samples collected from Korean chronic gastritis patients were used for this study. Twenty-three (41.1%) were positive for mycoplasmas. Eighteen sequenced samples contained a single human mycoplasma or two mycoplasmas, which were identified as Mycoplasma faucium (13/18), M. fermentans (3/18), M. orale (1/18), M. salivarium (2/18), and M. spermatophilum (1/ 18). Mycoplasma-infected chronic gastritis samples showed significantly more severe neutrophil infiltration than non-infected samples (P=0.0135). Mycoplasma profiles in the oral cavity (M. salivarium is major) and stomach were different, and the presence of significant proinflammatory responses in mycoplasma-positive patients suggests that the mycoplasmas are not simply contaminants. Further studies are required to understand whether mycoplasmas play a role in gastric tumorigenesis.",

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AU - Kang, Kyung Ah

AU - Kim, Jeong Ki

AU - Kang, Yoon Suk

AU - Song, Byung Cheol

AU - Kang, Hyun Wook

AU - Shim, Mi Ja

AU - Kim, Hee Sun

AU - Kim, Young Bae

AU - Hahm, Ki Baeg

AU - Kim, Bum Joon

AU - Kook, Myeong Cherl

AU - Chung, Myung Hee

AU - Hyun, Jin Won

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N2 - We aimed to determine whether mycoplasmas are present in Korean chronic gastritis, and to understand their roles in gastric cancer tumorigenesis, because mycoplasmas resemble Helicobacter pylori in terms of ammonia production and induction of inflammatory cytokines in immune and non-immune cells. The presence and identity of mycoplasmas were assessed by semi-nested PCR and sequencing, and the results were compared with pathologic data. Fifty-six samples collected from Korean chronic gastritis patients were used for this study. Twenty-three (41.1%) were positive for mycoplasmas. Eighteen sequenced samples contained a single human mycoplasma or two mycoplasmas, which were identified as Mycoplasma faucium (13/18), M. fermentans (3/18), M. orale (1/18), M. salivarium (2/18), and M. spermatophilum (1/ 18). Mycoplasma-infected chronic gastritis samples showed significantly more severe neutrophil infiltration than non-infected samples (P=0.0135). Mycoplasma profiles in the oral cavity (M. salivarium is major) and stomach were different, and the presence of significant proinflammatory responses in mycoplasma-positive patients suggests that the mycoplasmas are not simply contaminants. Further studies are required to understand whether mycoplasmas play a role in gastric tumorigenesis.

AB - We aimed to determine whether mycoplasmas are present in Korean chronic gastritis, and to understand their roles in gastric cancer tumorigenesis, because mycoplasmas resemble Helicobacter pylori in terms of ammonia production and induction of inflammatory cytokines in immune and non-immune cells. The presence and identity of mycoplasmas were assessed by semi-nested PCR and sequencing, and the results were compared with pathologic data. Fifty-six samples collected from Korean chronic gastritis patients were used for this study. Twenty-three (41.1%) were positive for mycoplasmas. Eighteen sequenced samples contained a single human mycoplasma or two mycoplasmas, which were identified as Mycoplasma faucium (13/18), M. fermentans (3/18), M. orale (1/18), M. salivarium (2/18), and M. spermatophilum (1/ 18). Mycoplasma-infected chronic gastritis samples showed significantly more severe neutrophil infiltration than non-infected samples (P=0.0135). Mycoplasma profiles in the oral cavity (M. salivarium is major) and stomach were different, and the presence of significant proinflammatory responses in mycoplasma-positive patients suggests that the mycoplasmas are not simply contaminants. Further studies are required to understand whether mycoplasmas play a role in gastric tumorigenesis.

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Presence of human mycoplasma DNA in gastric tissue samples from Korean chronic gastritis patients

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