Preparation of PLGA Nanoparticles by Milling Spongelike PLGA Microspheres

Jimin Lee, Hongkee Sah

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Currently, emulsification-templated nanoencapsulation techniques (e.g., nanoprecipitation) have been most frequently used to prepare poly-d,l-lactide-co-glycolide (PLGA) nanoparticles. This study aimed to explore a new top-down process to produce PLGA nanoparticles. The fundamental strategy was to prepare spongelike PLGA microspheres with a highly porous texture and then crush them into submicron-sized particles via wet milling. Therefore, an ethyl formate-based ammonolysis method was developed to encapsulate progesterone into porous PLGA microspheres. Compared to a conventional solvent evaporation process, the ammonolysis technique helped reduce the tendency of drug crystallization and improved drug encapsulation efficiency accordingly (solvent evaporation, 27.6 ± 4.6%; ammonolysis, 65.1 ± 1.7%). Wet milling was performed on the highly porous microspheres with a D50 of 64.8 μm under various milling conditions. The size of the grinding medium was the most crucial factor for our wet milling. Milling using smaller zirconium oxide beads (0.3~1 mm) was simply ineffective. However, when larger beads with diameters of 3 and 5 mm were used, our porous microspheres were ground into submicron-sized particles. The quality of the resultant PLGA nanoparticles was demonstrated by size distribution measurement and field emission scanning electron microscopy. The present top-down process that contrasts with conventional bottom-up approaches might find application in manufacturing drug-loaded PLGA nanoparticles.

Original languageEnglish
Article number1540
JournalPharmaceutics
Volume14
Issue number8
DOIs
StatePublished - Aug 2022

Bibliographical note

Funding Information:
This work was in part supported by Celros Biotech Co., Ltd., Seoul, Korea. The grant number is 2-2021-1896-001-1.

Publisher Copyright:
© 2022 by the authors.

Keywords

  • microspheres
  • nanoparticles
  • poly-d,l-lactide-co-glycolide
  • wet milling

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