Preparation and characterization of hyaluronic acid-based hydrogel nanoparticles

Ki Young Choi, Seulki Lee, Kyeongsoon Park, Kwangmeyung Kim, Jae Hyung Park, Ick Chan Kwon, Seo Young Jeong

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Novel polymeric amphiphilic hydrogel nanoparticles have been prepared by covalent attachment of hydrophobic tetradecylamine (TDA) to hyaluronic acid (HA) in the presence of 1-ethyl-3(3-dimethylaminopropyl)carbodiimide and N-hydroxysulfosuccinimide. Their chemical structure and self-association behavior in an aqueous solution were investigated by using 1H NMR, dynamic light scattering, fluorescence spectroscopy, and transmission electron microscopy (TEM). Owing to their amphiphilic characteristics, the resulting conjugates could form self-assembled hydrogel nanoparticles in an aqueous phase via the intra- or intermolecular association of hydrophobic moieties conjugated to the backbone of HA. From the TEM results, it was observed that the hydrogel nanoparticles were spherical in shape. The particle sizes and critical aggregation concentrations (CACs) of the conjugates were significantly dependent on the degree of substitution (DS) of TDA. The CACs of the conjugates were as low as 50-180 μg/mL; this suggests that the conjugates can form nanoparticles in diluted aqueous media such as body fluids. The mean diameters of the nanoparticles decreased as the DS of TDA increased, indicating that a larger amount of hydrophobic moieties in the conjugate allows formation of compact hydrophobic inner cores. It is anticipated that this HA-based nanoparticles can be used as new drug carriers for biomedical applications.

Original languageEnglish
Pages (from-to)1591-1595
Number of pages5
JournalJournal of Physics and Chemistry of Solids
Issue number5-6
StatePublished - May 2008

Bibliographical note

Funding Information:
This work was supported by the Ministry of Health and Welfare (A062254B8150506N11C011B), BK21 BNT Scientist Renovating for Drug Development Coping with Aged Society and by Seoul R&BD Program (10524M0214852) in Korea.


  • A. Polymers
  • B. Chemical synthesis
  • C. Electron microscopy


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