Pref-1 marks very early mesenchymal precursors required for adipose tissue development and expansion

Carolyn S. Hudak; Olga Gulyaeva; Yuhui Wang; Seung min Park; Luke Lee; Chulho Kang; Hei Sook Sul

doi:10.1016/j.celrep.2014.06.060

Pref-1 marks very early mesenchymal precursors required for adipose tissue development and expansion

Carolyn S. Hudak, Olga Gulyaeva, Yuhui Wang, Seung min Park, Luke Lee, Chulho Kang, Hei Sook Sul

Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

92 Scopus citations

Abstract

Pref-1 is an EGF-repeat-containing protein that inhibits adipocyte differentiation. To better understand the origin and development of white adipose tissue (WAT), we generated transgenic mouse models for transient or permanent fluorescent labeling of cells using the Pref-1 promoter, facilitating inducible ablation. We show that Pref-1-marked cells retain proliferative capacity and are very early adipose precursors, prior to expression of Zfp423 or PPARg. In addition, the Pref-1-marked cells establish that adipose precursors are mesenchymal, but not endothelial or pericytal, in origin. During embryogenesis, Pref-1-marked cells first appear in the dorsal mesenteric region as early as embryonic day 10.5 (E10.5). These cells become lipid-laden adipocytes at E17.5 in the subcutaneous region, whereas visceral WAT develops after birth. Finally, ablation of Pref-1- marked cells prevents not only embryonic WAT development but also later adult adipose expansion upon high-fat feeding, demonstrating the requirement of Pref-1 cells for adipogenesis.

Original language	English
Pages (from-to)	678-687
Number of pages	10
Journal	Cell Reports
Volume	8
Issue number	3
DOIs	https://doi.org/10.1016/j.celrep.2014.06.060
State	Published - 7 Aug 2014

Access to Document

10.1016/j.celrep.2014.06.060

Cite this

@article{91097fcadb304c12a99550e9bb3cbe53,

title = "Pref-1 marks very early mesenchymal precursors required for adipose tissue development and expansion",

abstract = "Pref-1 is an EGF-repeat-containing protein that inhibits adipocyte differentiation. To better understand the origin and development of white adipose tissue (WAT), we generated transgenic mouse models for transient or permanent fluorescent labeling of cells using the Pref-1 promoter, facilitating inducible ablation. We show that Pref-1-marked cells retain proliferative capacity and are very early adipose precursors, prior to expression of Zfp423 or PPARg. In addition, the Pref-1-marked cells establish that adipose precursors are mesenchymal, but not endothelial or pericytal, in origin. During embryogenesis, Pref-1-marked cells first appear in the dorsal mesenteric region as early as embryonic day 10.5 (E10.5). These cells become lipid-laden adipocytes at E17.5 in the subcutaneous region, whereas visceral WAT develops after birth. Finally, ablation of Pref-1- marked cells prevents not only embryonic WAT development but also later adult adipose expansion upon high-fat feeding, demonstrating the requirement of Pref-1 cells for adipogenesis.",

author = "Hudak, {Carolyn S.} and Olga Gulyaeva and Yuhui Wang and Park, {Seung min} and Luke Lee and Chulho Kang and Sul, {Hei Sook}",

note = "Funding Information: The authors are grateful to R. Duncan for assistance with transgenic mice generation; S. Ruzin and D. Schichnes of the Biological Imaging Facility, H. Nolla, and A. Valeros for assistance with FACS; J. Yuan, J. Chan, J. Dempersmier, and T. Tang for assistance; and J. Hudak for a critical reading of the manuscript. This work was supported by NIH grant DK50828 to H.S.S. C.S.H. was supported in part by NIH grant T32-DK61918. S.-m.P. was supported by NIH grant U54CA151459 (CCNE-T). Publisher Copyright: {\textcopyright} 2014 The Authors.",

year = "2014",

month = aug,

day = "7",

doi = "10.1016/j.celrep.2014.06.060",

language = "English",

volume = "8",

pages = "678--687",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Cell Press",

number = "3",

}

TY - JOUR

T1 - Pref-1 marks very early mesenchymal precursors required for adipose tissue development and expansion

AU - Hudak, Carolyn S.

AU - Gulyaeva, Olga

AU - Wang, Yuhui

AU - Park, Seung min

AU - Lee, Luke

AU - Kang, Chulho

AU - Sul, Hei Sook

N1 - Funding Information: The authors are grateful to R. Duncan for assistance with transgenic mice generation; S. Ruzin and D. Schichnes of the Biological Imaging Facility, H. Nolla, and A. Valeros for assistance with FACS; J. Yuan, J. Chan, J. Dempersmier, and T. Tang for assistance; and J. Hudak for a critical reading of the manuscript. This work was supported by NIH grant DK50828 to H.S.S. C.S.H. was supported in part by NIH grant T32-DK61918. S.-m.P. was supported by NIH grant U54CA151459 (CCNE-T). Publisher Copyright: © 2014 The Authors.

PY - 2014/8/7

Y1 - 2014/8/7

N2 - Pref-1 is an EGF-repeat-containing protein that inhibits adipocyte differentiation. To better understand the origin and development of white adipose tissue (WAT), we generated transgenic mouse models for transient or permanent fluorescent labeling of cells using the Pref-1 promoter, facilitating inducible ablation. We show that Pref-1-marked cells retain proliferative capacity and are very early adipose precursors, prior to expression of Zfp423 or PPARg. In addition, the Pref-1-marked cells establish that adipose precursors are mesenchymal, but not endothelial or pericytal, in origin. During embryogenesis, Pref-1-marked cells first appear in the dorsal mesenteric region as early as embryonic day 10.5 (E10.5). These cells become lipid-laden adipocytes at E17.5 in the subcutaneous region, whereas visceral WAT develops after birth. Finally, ablation of Pref-1- marked cells prevents not only embryonic WAT development but also later adult adipose expansion upon high-fat feeding, demonstrating the requirement of Pref-1 cells for adipogenesis.

AB - Pref-1 is an EGF-repeat-containing protein that inhibits adipocyte differentiation. To better understand the origin and development of white adipose tissue (WAT), we generated transgenic mouse models for transient or permanent fluorescent labeling of cells using the Pref-1 promoter, facilitating inducible ablation. We show that Pref-1-marked cells retain proliferative capacity and are very early adipose precursors, prior to expression of Zfp423 or PPARg. In addition, the Pref-1-marked cells establish that adipose precursors are mesenchymal, but not endothelial or pericytal, in origin. During embryogenesis, Pref-1-marked cells first appear in the dorsal mesenteric region as early as embryonic day 10.5 (E10.5). These cells become lipid-laden adipocytes at E17.5 in the subcutaneous region, whereas visceral WAT develops after birth. Finally, ablation of Pref-1- marked cells prevents not only embryonic WAT development but also later adult adipose expansion upon high-fat feeding, demonstrating the requirement of Pref-1 cells for adipogenesis.

UR - http://www.scopus.com/inward/record.url?scp=84923309352&partnerID=8YFLogxK

U2 - 10.1016/j.celrep.2014.06.060

DO - 10.1016/j.celrep.2014.06.060

M3 - Article

C2 - 25088414

AN - SCOPUS:84923309352

SN - 2211-1247

VL - 8

SP - 678

EP - 687

JO - Cell Reports

JF - Cell Reports

IS - 3

ER -

Pref-1 marks very early mesenchymal precursors required for adipose tissue development and expansion

Abstract

Access to Document

Fingerprint

Cite this