Precision protein assays on compartmentalized biosensor arrays

D. J.B. Bechstein; J. R. Lee; E. Ng; S. X. Wang

doi:10.1109/TRANSDUCERS.2015.7181255

Precision protein assays on compartmentalized biosensor arrays

D. J.B. Bechstein, J. R. Lee, E. Ng, S. X. Wang

Division of Mechanical and Biomedical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

A biological measurement microsystem enables precision protein assays by compartmentalization of biosensors in a sensor array. Using a PDMS microfluidic interface on an 8 by 8 Giant Magnetoresistive sensor array, this compartmentalization technique performs all required measurements, including biological references, on a single sensor chip alongside the actual sample(s) to be measured. All data is acquired simultaneously on a single chip, circumventing a range of possible errors currently present in sequential biosensor measurement approaches. With our approach, we achieve a low concentration estimation error of 11%. Additionally this compartmentalization technique enables high throughput measurements using multiple samples on a single chip with a large-scale array of solidstate sensors.

Original language	English
Title of host publication	2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	1637-1640
Number of pages	4
ISBN (Electronic)	9781479989553
DOIs	https://doi.org/10.1109/TRANSDUCERS.2015.7181255
State	Published - 5 Aug 2015
Event	18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 - Anchorage, United States Duration: 21 Jun 2015 → 25 Jun 2015

Publication series

Name	2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015

Conference

Conference	18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
Country/Territory	United States
City	Anchorage
Period	21/06/15 → 25/06/15

Bibliographical note

Publisher Copyright:
© 2015 IEEE.

Keywords

GMR
PDMS
Precision measurement
compartmentalization
immunoassay
magnetic
microfluidic
protein assay
sensor array
solid state

Access to Document

10.1109/TRANSDUCERS.2015.7181255

Cite this

Bechstein, D. J. B., Lee, J. R., Ng, E., & Wang, S. X. (2015). Precision protein assays on compartmentalized biosensor arrays. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 (pp. 1637-1640). Article 7181255 (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2015.7181255

Bechstein, D. J.B. ; Lee, J. R. ; Ng, E. et al. / Precision protein assays on compartmentalized biosensor arrays. 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc., 2015. pp. 1637-1640 (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015).

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abstract = "A biological measurement microsystem enables precision protein assays by compartmentalization of biosensors in a sensor array. Using a PDMS microfluidic interface on an 8 by 8 Giant Magnetoresistive sensor array, this compartmentalization technique performs all required measurements, including biological references, on a single sensor chip alongside the actual sample(s) to be measured. All data is acquired simultaneously on a single chip, circumventing a range of possible errors currently present in sequential biosensor measurement approaches. With our approach, we achieve a low concentration estimation error of 11%. Additionally this compartmentalization technique enables high throughput measurements using multiple samples on a single chip with a large-scale array of solidstate sensors.",

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Bechstein, DJB, Lee, JR, Ng, E & Wang, SX 2015, Precision protein assays on compartmentalized biosensor arrays. in 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015., 7181255, 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015, Institute of Electrical and Electronics Engineers Inc., pp. 1637-1640, 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015, Anchorage, United States, 21/06/15. https://doi.org/10.1109/TRANSDUCERS.2015.7181255

Precision protein assays on compartmentalized biosensor arrays. / Bechstein, D. J.B.; Lee, J. R.; Ng, E. et al.
2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc., 2015. p. 1637-1640 7181255 (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AB - A biological measurement microsystem enables precision protein assays by compartmentalization of biosensors in a sensor array. Using a PDMS microfluidic interface on an 8 by 8 Giant Magnetoresistive sensor array, this compartmentalization technique performs all required measurements, including biological references, on a single sensor chip alongside the actual sample(s) to be measured. All data is acquired simultaneously on a single chip, circumventing a range of possible errors currently present in sequential biosensor measurement approaches. With our approach, we achieve a low concentration estimation error of 11%. Additionally this compartmentalization technique enables high throughput measurements using multiple samples on a single chip with a large-scale array of solidstate sensors.

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Bechstein DJB, Lee JR, Ng E, Wang SX. Precision protein assays on compartmentalized biosensor arrays. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc. 2015. p. 1637-1640. 7181255. (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015). doi: 10.1109/TRANSDUCERS.2015.7181255

Precision protein assays on compartmentalized biosensor arrays

Abstract

Publication series

Conference

Bibliographical note

Keywords

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