Abstract
Alternariol monomethyl ether (AME), a dibenzopyrone derivative, was isolated from Alternaria brassicae along with altertoxin II (ATX-II). The compounds were tested for the inhibitory activity of monoamine oxidase (MAO), which catalyzes neurotransmitting monoamines. AME was found to be a highly potent and selective inhibitor of human MAO-A with an IC50 value of 1.71 µM; however, it was found to be ineffective for MAO-B inhibition. ATX-II was not effective for the inhibition of either MAO-A or MAO-B. The inhibition of MAO-A using AME was apparently instantaneous. MAO-A activity was almost completely recovered after the dilution of the inhibited enzyme with an excess amount of AME, suggesting AME is a reversible inhibitor. AME showed mixed inhibition for MAO-A in Lineweaver-Burk plots with a Ki value of 0.34 µM. The findings of this study suggest that microbial metabolites and dibenzopyronei could be potent MAO inhibitors. In addition, AME could be a useful lead compound for developing reversible MAO-A inhibitors to treat depression, Parkinson’s disease, and Alzheimer’s disease.
Original language | English |
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Pages (from-to) | 316-320 |
Number of pages | 5 |
Journal | Journal of Microbiology and Biotechnology |
Volume | 27 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2017 |
Bibliographical note
Publisher Copyright:© 2017 by The Korean Society for Microbiology and Biotechnology.
Keywords
- Alternaria brassicae
- Alternariol monomethyl ether
- Mixed inhibition
- Monoamine oxidase
- Selective inhibitor