TY - JOUR
T1 - Porphyrobacter algicida sp. nov., an algalytic bacterium isolated from seawater
AU - Kristyanto, Sylvia
AU - Lee, Sang Don
AU - Kim, Jaisoo
N1 - Funding Information:
This study was supported by National Research Foundation of Korea (NRF-2013M3A2A1067498) and by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (2016R1D1A1A09916982).
Publisher Copyright:
© 2017 IUMS.
PY - 2017/11
Y1 - 2017/11
N2 - A novel Gram-stain-negative, yellow-pigmented, catalase- and oxidase-positive, non-endospore-forming, flagellated bacterium, designated strain Yeonmyeong 2-22T, was isolated from surface seawater of Geoje Island, Republic of Korea. Strain Yeonmyeong 2-22T showed algalytic activity against the seven strains tested: Cochlodinium polykrikoides, Chattonella marina, Heterosigma akashiwo, Scrippsiella trochoidea, Heterocapsa triquetra, Prorocentrum minimum and Skeletonema costatum. A taxonomic study was carried out based on a polyphasic approach to characterize the exact taxonomic position of strain Yeonmyeong 2-22T. The bacterium was able to grow at 10–40 °C, at salinities from 0 to 9%, at pH from 4.0 to 9.0 and was not able to degrade gelatin or casein. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain Yeonmyeong 2-22T was considered to represent a novel species of the genus Porphyrobacter, which belongs to the family Erythrobacteraceae, and was related most closely to Porphyrobacter dokdonensis DSW-74T with 97.23% 16S rRNA gene sequence similarity. The dominant cellular fatty acids of strain Yeonmyeong 2-22T were C18: 1ω7c (49.7%), C16: 0 (12.0%) and 11-methyl C18: 1ω7c (11.5%), and ubiquinone-10 (Q-10) was the predominant respiratory lipoquinone. The genomic DNA G+C content of strain Yeonmyeong 2-22T was calculated to be 63.0 mol%. Phenotypic characteristics of the novel strain also differed from other members of the genus Porphyrobacter. On the basis of polyphasic taxonomic data, strain Yeonmyeong 2-22Trepresents as a novel species of the genus Porphyrobacter, for which the name of Porphyrobacter algicida sp. nov. is proposed. The type strain is Yeonmyeong 2-22T (=KEMB 9005-328T=JCM 31499T).
AB - A novel Gram-stain-negative, yellow-pigmented, catalase- and oxidase-positive, non-endospore-forming, flagellated bacterium, designated strain Yeonmyeong 2-22T, was isolated from surface seawater of Geoje Island, Republic of Korea. Strain Yeonmyeong 2-22T showed algalytic activity against the seven strains tested: Cochlodinium polykrikoides, Chattonella marina, Heterosigma akashiwo, Scrippsiella trochoidea, Heterocapsa triquetra, Prorocentrum minimum and Skeletonema costatum. A taxonomic study was carried out based on a polyphasic approach to characterize the exact taxonomic position of strain Yeonmyeong 2-22T. The bacterium was able to grow at 10–40 °C, at salinities from 0 to 9%, at pH from 4.0 to 9.0 and was not able to degrade gelatin or casein. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain Yeonmyeong 2-22T was considered to represent a novel species of the genus Porphyrobacter, which belongs to the family Erythrobacteraceae, and was related most closely to Porphyrobacter dokdonensis DSW-74T with 97.23% 16S rRNA gene sequence similarity. The dominant cellular fatty acids of strain Yeonmyeong 2-22T were C18: 1ω7c (49.7%), C16: 0 (12.0%) and 11-methyl C18: 1ω7c (11.5%), and ubiquinone-10 (Q-10) was the predominant respiratory lipoquinone. The genomic DNA G+C content of strain Yeonmyeong 2-22T was calculated to be 63.0 mol%. Phenotypic characteristics of the novel strain also differed from other members of the genus Porphyrobacter. On the basis of polyphasic taxonomic data, strain Yeonmyeong 2-22Trepresents as a novel species of the genus Porphyrobacter, for which the name of Porphyrobacter algicida sp. nov. is proposed. The type strain is Yeonmyeong 2-22T (=KEMB 9005-328T=JCM 31499T).
KW - Algalytic activity
KW - Harmful algal bloom
KW - Porphyrobacter algicida sp. nov
KW - Red tide
KW - Seawater
KW - Taxonomy
UR - http://www.scopus.com/inward/record.url?scp=85034268066&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.002324
DO - 10.1099/ijsem.0.002324
M3 - Article
C2 - 28984558
AN - SCOPUS:85034268066
SN - 1466-5026
VL - 67
SP - 4526
EP - 4533
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 11
ER -