TY - JOUR
T1 - Poly(Ethylene glycol)-poly(l-alanine)/hyaluronic acid complex as a 3d platform for understanding cancer cell migration in the tumor microenvironment
AU - Sim, Jooyoung
AU - Lee, Hyun Jung
AU - Jeong, Byeongmoon
AU - Park, Min Hee
N1 - Funding Information:
Funding: This work was supported by the National Research Foundation of Korea (NRF), grant funded by the Korea government (MSIT) (No. 2018R1C1B6002333).
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/4/1
Y1 - 2021/4/1
N2 - Cancer progression and migration in the tumor microenvironment are related to cell types and three-dimensional (3D) matrices. Therefore, developing biomimetic tumor models, including co-culture systems and a tunable 3D matrix, could play an essential role in understanding the cancer environment. Here, multicellular spheroids using human adipose-derived mesenchymal stem cells (hADSCs) and breast cancer cells (MDA-MB-231) within the 3D matrix were used as a tumor microenvironment (TME) mimicking platform. The amphiphilic peptide block copolymer and hyaluronic acid (HA) formed a self-assembled structure, which provides a biocompatible 3D environment for the cells. Multicellular spheroids were formed on the optimized plate and were observed as cell migration from a spheroid within a 3D matrix, such as the invasive and metastatic cancer of TME. This study suggests a new 3D platform using polymer complexes and the importance of tumor complexities, including various cell types and microenvironments.
AB - Cancer progression and migration in the tumor microenvironment are related to cell types and three-dimensional (3D) matrices. Therefore, developing biomimetic tumor models, including co-culture systems and a tunable 3D matrix, could play an essential role in understanding the cancer environment. Here, multicellular spheroids using human adipose-derived mesenchymal stem cells (hADSCs) and breast cancer cells (MDA-MB-231) within the 3D matrix were used as a tumor microenvironment (TME) mimicking platform. The amphiphilic peptide block copolymer and hyaluronic acid (HA) formed a self-assembled structure, which provides a biocompatible 3D environment for the cells. Multicellular spheroids were formed on the optimized plate and were observed as cell migration from a spheroid within a 3D matrix, such as the invasive and metastatic cancer of TME. This study suggests a new 3D platform using polymer complexes and the importance of tumor complexities, including various cell types and microenvironments.
KW - 3D matrix
KW - Migration
KW - Multicellular spheroid
KW - Polymer complex
KW - Tumor microenvironment
UR - http://www.scopus.com/inward/record.url?scp=85103982020&partnerID=8YFLogxK
U2 - 10.3390/polym13071042
DO - 10.3390/polym13071042
M3 - Article
AN - SCOPUS:85103982020
SN - 2073-4360
VL - 13
JO - Polymers
JF - Polymers
IS - 7
M1 - 1042
ER -