Abstract
We demonstrate that gold nanoparticles (GNPs) with self-assembled monolayer (SAM) can function as all-optical nanoscale biomolecular detectors that monitor in situ resonant biomolecules, such as Cytochrome c. By tethering resonant biomolecules to Au nanoprobes, the binding events of Cytochrome c onto 50-nm Au nanoprobes yield characteristic spectral dips in scattering spectra of Au nanoprobes due to plasmon resonance energy transfer (PRET). The quenching dip depth of the single nanoprobe spectrum is found to be a function of incubation time (t) and concentration of Cytochrome c, which provides a real-time and nanoscale measurement and quantification of Cytochrome c.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 |
| Editors | Jean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1610-1612 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806407 |
| State | Published - 2007 |
| Event | 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, France Duration: 7 Oct 2007 → 11 Oct 2007 |
Publication series
| Name | Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 |
|---|
Conference
| Conference | 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 |
|---|---|
| Country/Territory | France |
| City | Paris |
| Period | 7/10/07 → 11/10/07 |
Bibliographical note
Publisher Copyright:© 2007 CBMS.
Keywords
- Cytochrome C
- Gold Nanoparticle
- Plasmonic Resonance Energy Transfer
- Rayleigh Scattering