Plasmacytoid dendritic cells contribute to the production of ifn-β via tlr7-myd88-dependent pathway and ctl priming during respiratory syncytial virus infection

Tae Hoon Kim, Dong Sun Oh, Hi Eun Jung, Jun Chang, Heung Kyu Lee

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Respiratory syncytial virus (RSV) is the leading cause of respiratory viral infection in infants and children, yet little is known about the antiviral response of plasmacytoid dendritic cells (pDCs) to RSV infection. We tracked the cellular source of interferon-β using interferon-β/yellow fluorescent protein (YFP) reporter mice and identified the signaling pathway activated by RSV that induces type I interferon production in pDCs and DCs. Results from in vitro analyses of RSV-stimulated bone marrow cells revealed that RSV induces interferon-β production in both pDCs and DCs. Kinetic analyses of interferon-β-producing cells in RSV-infected lung cells in vivo indicated that pDCs are rapidly recruited to sites of inflammation during infection. These cells produced interferon-β via the TLR7-MyD88-mediated pathway and IFNα1R-mediated pathway rather than the MAVS-mediated pathway. Moreover, pDC-ablated mice exhibited decreased interferon-γ production and the antigen specificity of CD8+ T cells. Collectively, these data indicate that pDCs play pivotal roles in cytotoxic T lymphocyte (CTL) responses and are one of producers of type I interferon during RSV infection.

Original languageEnglish
Article number730
JournalViruses
Volume11
Issue number8
DOIs
StatePublished - Aug 2019

Keywords

  • Cytotoxic CD8 T cells
  • Dendritic cell
  • Plasmacytoid dendritic cell
  • RSV
  • Type I IFN

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