In this work, the use of scaffolds is reported, templated from live neurons as an advanced culture platform for primary neurons. Hippocampal neurons cultured on neurotemplated scaffolds exhibit an affinity for templated somas, revealing a preference for micrometric structures amidst nanotopographical features. It is also reported, for the first time, that neurite complexity can be topographically controlled by increasing the density of nanometric features on neurotemplated scaffolds. Neurotemplated scaffolds are versatile, hierarchical topographies that feature biologically relevant structures, in both form and scale, and capture the true complexity of an in vivo environment. The introduction and implementation of neurotemplated scaffolds is sure to advance research in the fields of neurodevelopment, network development, and neuroregeneration.
Bibliographical noteFunding Information:
B.J.K. and M.P. contributed equally to this work. This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (MSIP) (2012R1A3A2026403). This study was approved by the Institutional Animal Care and Use Committee (IACUC) of KAIST.
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- axonal pathfinding
- hierarchical topographies
- neurite complexity
- neurotemplated scaffolds
- pioneer neurons