TY - JOUR
T1 - Pinitol consumption improves liver health status by reducing oxidative stress and fatty acid accumulation in subjects with non-alcoholic fatty liver disease
T2 - A randomized, double-blind, placebo-controlled trial
AU - Lee, Eunok
AU - Lim, Yeni
AU - Kwon, Sung Won
AU - Kwon, Oran
N1 - Publisher Copyright:
© 2019 The Authors
PY - 2019/6
Y1 - 2019/6
N2 - Non-alcoholic fatty liver disease (NAFLD) is the most prevalent cause of chronic hepatic injury in the world. One of the most important therapeutic strategies for this disease is modulating oxidative stress. This study hypothesized that supplementation of pinitol might exert hepatic protective effects, by modulating oxidative stress in subjects with NAFLD. A randomized, double-blind controlled trial was conducted in 90 subjects with ultrasonography-proven NAFLD, who were randomly assigned to the placebo, low-dose (300 mg/d), or high-dose (500 mg/d) of pinitol for 12 weeks. The outcome measures were liver fat content, liver enzymes, fasting and postprandial lipids, and oxidative stress levels. To understand the underlying mechanism, plasma metabolomic analysis based on a gas chromatography/time-of-flight mass spectrometry and urinary pinitol analysis were also performed. The pinitol group showed significantly lower levels in liver fat content, plasma liver enzymes, fasting/postprandial urinary malondialdehyde levels, and postprandial triglycerides concentrations, but significantly higher in glutathione peroxidase level compared with the placebo group. The metabolomic analysis identified 27 differential metabolites involved in glycine/serine/threonine metabolism, alanine/aspartate/glutamate metabolism, D-glutamine/D-glutamate metabolism, and fatty acid synthesis, implicating the role of pinitol in glutathione-related lipid and energy metabolism. These results suggest that pinitol may exert modulatory effects upon energy and metabolic pathways by reducing oxidative stress and fatty acid accumulation, which can lead to hepatoprotective benefits in NAFLD subjects.
AB - Non-alcoholic fatty liver disease (NAFLD) is the most prevalent cause of chronic hepatic injury in the world. One of the most important therapeutic strategies for this disease is modulating oxidative stress. This study hypothesized that supplementation of pinitol might exert hepatic protective effects, by modulating oxidative stress in subjects with NAFLD. A randomized, double-blind controlled trial was conducted in 90 subjects with ultrasonography-proven NAFLD, who were randomly assigned to the placebo, low-dose (300 mg/d), or high-dose (500 mg/d) of pinitol for 12 weeks. The outcome measures were liver fat content, liver enzymes, fasting and postprandial lipids, and oxidative stress levels. To understand the underlying mechanism, plasma metabolomic analysis based on a gas chromatography/time-of-flight mass spectrometry and urinary pinitol analysis were also performed. The pinitol group showed significantly lower levels in liver fat content, plasma liver enzymes, fasting/postprandial urinary malondialdehyde levels, and postprandial triglycerides concentrations, but significantly higher in glutathione peroxidase level compared with the placebo group. The metabolomic analysis identified 27 differential metabolites involved in glycine/serine/threonine metabolism, alanine/aspartate/glutamate metabolism, D-glutamine/D-glutamate metabolism, and fatty acid synthesis, implicating the role of pinitol in glutathione-related lipid and energy metabolism. These results suggest that pinitol may exert modulatory effects upon energy and metabolic pathways by reducing oxidative stress and fatty acid accumulation, which can lead to hepatoprotective benefits in NAFLD subjects.
KW - Liver enzyme
KW - Liver fat content
KW - Non-alcoholic fatty liver disease
KW - Oxidative stress
KW - Pinitol
UR - http://www.scopus.com/inward/record.url?scp=85064697690&partnerID=8YFLogxK
U2 - 10.1016/j.jnutbio.2019.03.006
DO - 10.1016/j.jnutbio.2019.03.006
M3 - Article
C2 - 31030165
AN - SCOPUS:85064697690
SN - 0955-2863
VL - 68
SP - 33
EP - 41
JO - Journal of Nutritional Biochemistry
JF - Journal of Nutritional Biochemistry
ER -