Abstract
Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB/V)], by fed-batch culture of recombinant Escherichia coli harboring a plasmid containing the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes, was examined in two pilot-scale fermentors with air supply only. In a 30 L fermentor having a KLa value of 0.11 s-1, the final P(3HB/V) concentration and the P(3HB/V) content obtained were 29.6 g/L and 70.1 wt%, respectively, giving a productivity of 1.37 g P(3HB/V)/L-h. In a 300 L fermentor having a KLa of 0.03 s-1, the P(3HB/V) concentration and the P(3HB/V) content were 20.4 g/L and 69 wt%, respectively, giving a productivity of 1.06 g P(3HB/V)/L-h. These results suggest that economical production of P(3HB/V) is possible by fed-batch culture of recombinant E. coli in a large-scale fermentor having low KLa value.
Original language | English |
---|---|
Pages (from-to) | 371-374 |
Number of pages | 4 |
Journal | Biotechnology and Bioprocess Engineering |
Volume | 7 |
Issue number | 6 |
DOIs | |
State | Published - 2002 |
Keywords
- Oxygen transfer rate
- P(3HB/V)
- Pilot-scale fermentor
- Recombinant E. coli