Pilot scale production of pory(3-hydroxybutyrate-co-3-hydroxyvalerate) by fed-batch culture of recombinant Escherichia coli

Jong Il Choi, Sang Yup Lee, Kyungsup Shin, Woo Gi Lee, Si Jae Park, Ho Nam Chang, Yong Keun Chang

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB/V)], by fed-batch culture of recombinant Escherichia coli harboring a plasmid containing the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes, was examined in two pilot-scale fermentors with air supply only. In a 30 L fermentor having a KLa value of 0.11 s-1, the final P(3HB/V) concentration and the P(3HB/V) content obtained were 29.6 g/L and 70.1 wt%, respectively, giving a productivity of 1.37 g P(3HB/V)/L-h. In a 300 L fermentor having a KLa of 0.03 s-1, the P(3HB/V) concentration and the P(3HB/V) content were 20.4 g/L and 69 wt%, respectively, giving a productivity of 1.06 g P(3HB/V)/L-h. These results suggest that economical production of P(3HB/V) is possible by fed-batch culture of recombinant E. coli in a large-scale fermentor having low KLa value.

Original languageEnglish
Pages (from-to)371-374
Number of pages4
JournalBiotechnology and Bioprocess Engineering
Volume7
Issue number6
DOIs
StatePublished - 2002

Keywords

  • Oxygen transfer rate
  • P(3HB/V)
  • Pilot-scale fermentor
  • Recombinant E. coli

Fingerprint

Dive into the research topics of 'Pilot scale production of pory(3-hydroxybutyrate-co-3-hydroxyvalerate) by fed-batch culture of recombinant Escherichia coli'. Together they form a unique fingerprint.

Cite this