Piezoelectric olfactory biosensor: Ligand specificity and dose-dependence of an olfactory receptor expressed in a heterologous cell system

Hwi Jin Ko; Tai Hyun Park

doi:10.1016/j.bios.2004.05.002

Piezoelectric olfactory biosensor: Ligand specificity and dose-dependence of an olfactory receptor expressed in a heterologous cell system

Hwi Jin Ko, Tai Hyun Park

Department of Nutritional Science & Food Management

Research output: Contribution to journal › Article › peer-review

100 Scopus citations

Abstract

An olfactory receptor protein of rats, I7, was expressed on the surface of human embryonic kidney (HEK)-293 cells. For targeting and detecting the protein, rho-tag import sequence was fused with the I7 protein. The olfactory receptor was expressed on the plasma membrane of HEK-293 cells, and stable cell lines regulated by an inducer were obtained. The expression on the cell surface was confirmed by immunocytochemical and Western blotting methods, and the binding of specific odorant molecules to the olfactory receptor was measured using quartz crystal microbalance (QCM). The results for QCM coated with cells containing the olfactory receptor showed that the expressed protein I7 strongly interacted with octyl aldehyde (octanal), which is an odorant specific to the I7 protein. Several other odorants were tested, and the results showed that I7 interacted differently with them. The QCM response to the serial concentrations of octyl aldehyde showed that the response is dose dependent. All these results indicate that the I7 receptor protein expressed on the surface of the heterologous cell system is sensitive to the specific odorant and can be used for the quantitative measurement of the odorant.

Original language	English
Pages (from-to)	1327-1332
Number of pages	6
Journal	Biosensors and Bioelectronics
Volume	20
Issue number	7
DOIs	https://doi.org/10.1016/j.bios.2004.05.002
State	Published - 15 Jan 2005

Bibliographical note

Funding Information:
This work was supported by the Korea Science & Engineering Foundation through the Nano-Bioelectronics and Systems Research Center, Seoul National University, Seoul Korea. We thank Randall R. Reed (Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA) for the rho-tag gene, Akihito Yasuoka (Bio-oriented Technology Research Advancement Institution, Saitama, Japan) for the pVL-I7, and Paul A. Hargrave (J. Hillis Miller Health Center, Department of Ophthalmology, University of Florida College of Medicine, Gainesville, FL, USA) for the B6-30 cell line.

Keywords

HEK-293 cell
Octyl aldehyde
Olfactory receptor protein
Quartz crystal microbalance

Access to Document

10.1016/j.bios.2004.05.002

Cite this

@article{8695052534c2484f96a14630ab134e1b,

title = "Piezoelectric olfactory biosensor: Ligand specificity and dose-dependence of an olfactory receptor expressed in a heterologous cell system",

abstract = "An olfactory receptor protein of rats, I7, was expressed on the surface of human embryonic kidney (HEK)-293 cells. For targeting and detecting the protein, rho-tag import sequence was fused with the I7 protein. The olfactory receptor was expressed on the plasma membrane of HEK-293 cells, and stable cell lines regulated by an inducer were obtained. The expression on the cell surface was confirmed by immunocytochemical and Western blotting methods, and the binding of specific odorant molecules to the olfactory receptor was measured using quartz crystal microbalance (QCM). The results for QCM coated with cells containing the olfactory receptor showed that the expressed protein I7 strongly interacted with octyl aldehyde (octanal), which is an odorant specific to the I7 protein. Several other odorants were tested, and the results showed that I7 interacted differently with them. The QCM response to the serial concentrations of octyl aldehyde showed that the response is dose dependent. All these results indicate that the I7 receptor protein expressed on the surface of the heterologous cell system is sensitive to the specific odorant and can be used for the quantitative measurement of the odorant.",

keywords = "HEK-293 cell, Octyl aldehyde, Olfactory receptor protein, Quartz crystal microbalance",

author = "Ko, {Hwi Jin} and Park, {Tai Hyun}",

note = "Funding Information: This work was supported by the Korea Science & Engineering Foundation through the Nano-Bioelectronics and Systems Research Center, Seoul National University, Seoul Korea. We thank Randall R. Reed (Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA) for the rho-tag gene, Akihito Yasuoka (Bio-oriented Technology Research Advancement Institution, Saitama, Japan) for the pVL-I7, and Paul A. Hargrave (J. Hillis Miller Health Center, Department of Ophthalmology, University of Florida College of Medicine, Gainesville, FL, USA) for the B6-30 cell line. ",

year = "2005",

month = jan,

day = "15",

doi = "10.1016/j.bios.2004.05.002",

language = "English",

volume = "20",

pages = "1327--1332",

journal = "Biosensors and Bioelectronics",

issn = "0956-5663",

publisher = "Elsevier Ltd.",

number = "7",

}

TY - JOUR

T1 - Piezoelectric olfactory biosensor

T2 - Ligand specificity and dose-dependence of an olfactory receptor expressed in a heterologous cell system

AU - Ko, Hwi Jin

AU - Park, Tai Hyun

N1 - Funding Information: This work was supported by the Korea Science & Engineering Foundation through the Nano-Bioelectronics and Systems Research Center, Seoul National University, Seoul Korea. We thank Randall R. Reed (Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA) for the rho-tag gene, Akihito Yasuoka (Bio-oriented Technology Research Advancement Institution, Saitama, Japan) for the pVL-I7, and Paul A. Hargrave (J. Hillis Miller Health Center, Department of Ophthalmology, University of Florida College of Medicine, Gainesville, FL, USA) for the B6-30 cell line.

PY - 2005/1/15

Y1 - 2005/1/15

N2 - An olfactory receptor protein of rats, I7, was expressed on the surface of human embryonic kidney (HEK)-293 cells. For targeting and detecting the protein, rho-tag import sequence was fused with the I7 protein. The olfactory receptor was expressed on the plasma membrane of HEK-293 cells, and stable cell lines regulated by an inducer were obtained. The expression on the cell surface was confirmed by immunocytochemical and Western blotting methods, and the binding of specific odorant molecules to the olfactory receptor was measured using quartz crystal microbalance (QCM). The results for QCM coated with cells containing the olfactory receptor showed that the expressed protein I7 strongly interacted with octyl aldehyde (octanal), which is an odorant specific to the I7 protein. Several other odorants were tested, and the results showed that I7 interacted differently with them. The QCM response to the serial concentrations of octyl aldehyde showed that the response is dose dependent. All these results indicate that the I7 receptor protein expressed on the surface of the heterologous cell system is sensitive to the specific odorant and can be used for the quantitative measurement of the odorant.

AB - An olfactory receptor protein of rats, I7, was expressed on the surface of human embryonic kidney (HEK)-293 cells. For targeting and detecting the protein, rho-tag import sequence was fused with the I7 protein. The olfactory receptor was expressed on the plasma membrane of HEK-293 cells, and stable cell lines regulated by an inducer were obtained. The expression on the cell surface was confirmed by immunocytochemical and Western blotting methods, and the binding of specific odorant molecules to the olfactory receptor was measured using quartz crystal microbalance (QCM). The results for QCM coated with cells containing the olfactory receptor showed that the expressed protein I7 strongly interacted with octyl aldehyde (octanal), which is an odorant specific to the I7 protein. Several other odorants were tested, and the results showed that I7 interacted differently with them. The QCM response to the serial concentrations of octyl aldehyde showed that the response is dose dependent. All these results indicate that the I7 receptor protein expressed on the surface of the heterologous cell system is sensitive to the specific odorant and can be used for the quantitative measurement of the odorant.

KW - HEK-293 cell

KW - Octyl aldehyde

KW - Olfactory receptor protein

KW - Quartz crystal microbalance

UR - http://www.scopus.com/inward/record.url?scp=10044278974&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2004.05.002

DO - 10.1016/j.bios.2004.05.002

M3 - Article

C2 - 15590286

AN - SCOPUS:10044278974

SN - 0956-5663

VL - 20

SP - 1327

EP - 1332

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 7

ER -

Piezoelectric olfactory biosensor: Ligand specificity and dose-dependence of an olfactory receptor expressed in a heterologous cell system

Abstract

Bibliographical note

Keywords

Access to Document

Fingerprint

Cite this