Piezoelectric biosensor using olfactory receptor protein expressed in Escherichia coli

Jong Hwan Sung, Hwi Jin Ko, Tai Hyun Park

Research output: Contribution to journalArticlepeer-review

100 Scopus citations

Abstract

An olfactory receptor protein of C. elegans, ODR-10, was expressed in Escherichia coli as a fusion protein, with GST and 6× His-tag. The expression of the target protein was analyzed by SDS-PAGE and Western blot, and was confirmed to be expressed at the membrane fraction of the host E. coli. The surface of a quartz crystal microbalance (QCM) was coated with crude membrane extracts, containing the expressed receptor protein, and the interaction between the olfactory receptor and various odorant molecules examined. Compared with other odorants, diacetyl (2,3-butanedione), known as a natural ligand for the ODR-10 receptor, interacted most strongly with the expressed protein. Various concentrations of diacetyl were applied to the expressed ODR-10 receptor, and the response of the QCM showed a linear relationship to the logarithmic value of the odorant concentration. This piezoelectric biosensor system, using olfactory receptor proteins expressed in E. coli, can be used in diagnostics, toxic chemical detection and the quality control of food.

Original languageEnglish
Pages (from-to)1981-1986
Number of pages6
JournalBiosensors and Bioelectronics
Volume21
Issue number10
DOIs
StatePublished - 15 Apr 2006

Bibliographical note

Funding Information:
This work was supported by the Korea Science & Engineering Foundation through the Nano-Bioelectronics and Systems Research Center, Seoul National University, Seoul Korea. We thank Cornelia I. Bargmann (Department of Anatomy, University of California, San Francisco, CA, USA) for the odr-10 gene.

Keywords

  • C. elegans
  • E. coli
  • ODR-10
  • Olfactory receptor proteins
  • Quartz crystal microbalance

Fingerprint

Dive into the research topics of 'Piezoelectric biosensor using olfactory receptor protein expressed in Escherichia coli'. Together they form a unique fingerprint.

Cite this