Piconewton regime measurements of biomolecular interactions by nanomechanical force gauge

Ki Hun Jeong; M. Pio; C. G. Keller; L. P. Lee

doi:10.1109/NANO.2002.1032149

Piconewton regime measurements of biomolecular interactions by nanomechanical force gauge

Ki Hun Jeong, M. Pio, C. G. Keller, L. P. Lee

Department of Chemistry & Nanoscience

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

A piconewton regime measurement of biomolecular interactions in an aqueous solution by a novel nanomechanical force gauge (NFG) is presented in this paper. A highly sensitive nanoscale cantilever with a spring constant, which is thousand times smaller than that of an atomic force microscope (AFM) microcantilever, is fabricated by a batch process. The NFG has a capability of direct reading without any optical amplification. The control of nanoscale thickness of a single crystal silicon cantilever is done by a thermal oxidation process. The deflection of the cantilever, corresponding to piconewtons is directly measured by reading the tick movements in the reading scale of the NFG under the microscope. The spring constant of the NFG is calculated by identifying the natural frequency using electrostatic force excitation, and the minimum value of the designed device was 78.6 pN/μm. As an example of the biomolecular applications, the dissociation between a biotinylated bead and avidins is measured, and the mean is 636 pN. The NFG has the potential of 1 pN/μm sensitivity through the nanofabrication technology as well as serving as an inexpensive and powerful substitute for an atomic force microscope in studying biomolecular interactions.

Original language	English
Title of host publication	Proceedings of the 2002 2nd IEEE Conference on Nanotechnology, IEEE-NANO 2002
Publisher	IEEE Computer Society
Pages	129-132
Number of pages	4
ISBN (Electronic)	0780375386
DOIs	https://doi.org/10.1109/NANO.2002.1032149
State	Published - 2002
Event	2nd IEEE Conference on Nanotechnology, IEEE-NANO 2002 - Washington, United States Duration: 26 Aug 2002 → 28 Aug 2002

Publication series

Name	Proceedings of the IEEE Conference on Nanotechnology
Volume	2002-January
ISSN (Print)	1944-9399
ISSN (Electronic)	1944-9380

Conference

Conference	2nd IEEE Conference on Nanotechnology, IEEE-NANO 2002
Country/Territory	United States
City	Washington
Period	26/08/02 → 28/08/02

Bibliographical note

Publisher Copyright:
© 2002 IEEE.

Keywords

Atomic force microscopy
Atomic measurements
Electrostatic measurements
Force measurement
Frequency
Oxidation
Silicon
Springs
Stimulated emission
Thickness control

Access to Document

10.1109/NANO.2002.1032149

Cite this

Jeong, K. H., Pio, M., Keller, C. G., & Lee, L. P. (2002). Piconewton regime measurements of biomolecular interactions by nanomechanical force gauge. In Proceedings of the 2002 2nd IEEE Conference on Nanotechnology, IEEE-NANO 2002 (pp. 129-132). Article 1032149 (Proceedings of the IEEE Conference on Nanotechnology; Vol. 2002-January). IEEE Computer Society. https://doi.org/10.1109/NANO.2002.1032149

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title = "Piconewton regime measurements of biomolecular interactions by nanomechanical force gauge",

abstract = "A piconewton regime measurement of biomolecular interactions in an aqueous solution by a novel nanomechanical force gauge (NFG) is presented in this paper. A highly sensitive nanoscale cantilever with a spring constant, which is thousand times smaller than that of an atomic force microscope (AFM) microcantilever, is fabricated by a batch process. The NFG has a capability of direct reading without any optical amplification. The control of nanoscale thickness of a single crystal silicon cantilever is done by a thermal oxidation process. The deflection of the cantilever, corresponding to piconewtons is directly measured by reading the tick movements in the reading scale of the NFG under the microscope. The spring constant of the NFG is calculated by identifying the natural frequency using electrostatic force excitation, and the minimum value of the designed device was 78.6 pN/μm. As an example of the biomolecular applications, the dissociation between a biotinylated bead and avidins is measured, and the mean is 636 pN. The NFG has the potential of 1 pN/μm sensitivity through the nanofabrication technology as well as serving as an inexpensive and powerful substitute for an atomic force microscope in studying biomolecular interactions.",

keywords = "Atomic force microscopy, Atomic measurements, Electrostatic measurements, Force measurement, Frequency, Oxidation, Silicon, Springs, Stimulated emission, Thickness control",

author = "Jeong, {Ki Hun} and M. Pio and Keller, {C. G.} and Lee, {L. P.}",

note = "Publisher Copyright: {\textcopyright} 2002 IEEE.; 2nd IEEE Conference on Nanotechnology, IEEE-NANO 2002 ; Conference date: 26-08-2002 Through 28-08-2002",

year = "2002",

doi = "10.1109/NANO.2002.1032149",

language = "English",

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Jeong, KH, Pio, M, Keller, CG & Lee, LP 2002, Piconewton regime measurements of biomolecular interactions by nanomechanical force gauge. in Proceedings of the 2002 2nd IEEE Conference on Nanotechnology, IEEE-NANO 2002., 1032149, Proceedings of the IEEE Conference on Nanotechnology, vol. 2002-January, IEEE Computer Society, pp. 129-132, 2nd IEEE Conference on Nanotechnology, IEEE-NANO 2002, Washington, United States, 26/08/02. https://doi.org/10.1109/NANO.2002.1032149

Piconewton regime measurements of biomolecular interactions by nanomechanical force gauge. / Jeong, Ki Hun; Pio, M.; Keller, C. G. et al.
Proceedings of the 2002 2nd IEEE Conference on Nanotechnology, IEEE-NANO 2002. IEEE Computer Society, 2002. p. 129-132 1032149 (Proceedings of the IEEE Conference on Nanotechnology; Vol. 2002-January).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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N2 - A piconewton regime measurement of biomolecular interactions in an aqueous solution by a novel nanomechanical force gauge (NFG) is presented in this paper. A highly sensitive nanoscale cantilever with a spring constant, which is thousand times smaller than that of an atomic force microscope (AFM) microcantilever, is fabricated by a batch process. The NFG has a capability of direct reading without any optical amplification. The control of nanoscale thickness of a single crystal silicon cantilever is done by a thermal oxidation process. The deflection of the cantilever, corresponding to piconewtons is directly measured by reading the tick movements in the reading scale of the NFG under the microscope. The spring constant of the NFG is calculated by identifying the natural frequency using electrostatic force excitation, and the minimum value of the designed device was 78.6 pN/μm. As an example of the biomolecular applications, the dissociation between a biotinylated bead and avidins is measured, and the mean is 636 pN. The NFG has the potential of 1 pN/μm sensitivity through the nanofabrication technology as well as serving as an inexpensive and powerful substitute for an atomic force microscope in studying biomolecular interactions.

AB - A piconewton regime measurement of biomolecular interactions in an aqueous solution by a novel nanomechanical force gauge (NFG) is presented in this paper. A highly sensitive nanoscale cantilever with a spring constant, which is thousand times smaller than that of an atomic force microscope (AFM) microcantilever, is fabricated by a batch process. The NFG has a capability of direct reading without any optical amplification. The control of nanoscale thickness of a single crystal silicon cantilever is done by a thermal oxidation process. The deflection of the cantilever, corresponding to piconewtons is directly measured by reading the tick movements in the reading scale of the NFG under the microscope. The spring constant of the NFG is calculated by identifying the natural frequency using electrostatic force excitation, and the minimum value of the designed device was 78.6 pN/μm. As an example of the biomolecular applications, the dissociation between a biotinylated bead and avidins is measured, and the mean is 636 pN. The NFG has the potential of 1 pN/μm sensitivity through the nanofabrication technology as well as serving as an inexpensive and powerful substitute for an atomic force microscope in studying biomolecular interactions.

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KW - Atomic measurements

KW - Electrostatic measurements

KW - Force measurement

KW - Frequency

KW - Oxidation

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KW - Springs

KW - Stimulated emission

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T3 - Proceedings of the IEEE Conference on Nanotechnology

SP - 129

EP - 132

BT - Proceedings of the 2002 2nd IEEE Conference on Nanotechnology, IEEE-NANO 2002

PB - IEEE Computer Society

T2 - 2nd IEEE Conference on Nanotechnology, IEEE-NANO 2002

Y2 - 26 August 2002 through 28 August 2002

ER -

Piconewton regime measurements of biomolecular interactions by nanomechanical force gauge

Abstract

Publication series

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Bibliographical note

Keywords

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