Phytosphingosine promotes megakaryocytic differentiation of myeloid leukemia cells

Sang Hee Han, Jusong Kim, Yerim Her, Ikjoo Seong, Sera Park, Deepak Bhattarai, Guanghai Jin, Kyeong Lee, Gukhoon Chung, Sungkee Hwang, Yun Soo Bae, Jaesang Kim

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

We report that phytosphingosine, a sphingolipid found in many organisms and implicated in cellular signaling, promotes megakaryocytic differentiation of myeloid leukemia cells. Specifically, phytosphingosine induced several hallmark changes associated with megakaryopoiesis from K562 and HEL cells including cell cycle arrest, cell size increase and polyploidization. We also confirmed that cell type specific markers of megakaryocytes, CD41a and CD42b are induced by phytosphingosine. Phospholipids with highly similar structures were unable to induce similar changes, indicating that the activity of phytosphingosine is highly specific. Although phytosphingosine is known to activate p38 mitogen-activated protein kinase (MAPK)-mediated apoptosis, the signaling mechanisms involved in megakaryopoiesis appear to be distinct. In sum, we present another model for dissecting molecular details of megakaryocytic differentiation which in large part remains obscure.

Original languageEnglish
Pages (from-to)691-695
Number of pages5
JournalBMB Reports
Volume48
Issue number12
DOIs
StatePublished - 2015

Keywords

  • (R)-TEMOSPho
  • K562
  • Megakaryocyte
  • Phytosphingosine
  • Platelet

Fingerprint

Dive into the research topics of 'Phytosphingosine promotes megakaryocytic differentiation of myeloid leukemia cells'. Together they form a unique fingerprint.

Cite this