Phylogeography of Mytilisepta virgata (Mytilidae: Bivalvia) in the northwestern Pacific: Cryptic mitochondrial lineages and mito-nuclear discordance

Yucheol Lee, Gang Ni, Jinkyung Shin, Taeho Kim, Elizabeth M.A. Kern, Yuseob Kim, Seung Chul Kim, Benny Chan, Ryutaro Goto, Tomoyuki Nakano, Joong Ki Park

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


The purplish bifurcate mussel Mytilisepta virgata is widely distributed and represents one of the major components of the intertidal community in the northwestern Pacific (NWP). Here, we characterized population genetic structure of NWP populations throughout nearly their whole distribution range using both mitochondrial (mtDNA cox1) and nuclear (ITS1) markers. Population genetic analyses for mtDNA cox 1 sequences revealed two monophyletic lineages (i.e., southern and northern lineages) geographically distributed according to the two different surface water temperature zones in the NWP. The timing of the lineage split is estimated at the Pliocene- mid-Pleistocene (5.49–1.61 Mya), which is consistent with the timing of the historical isolation of the East Sea/Sea of Japan from the South and East China Seas due to sea level decline during glacial cycles. Historical sea level fluctuation during the Pliocene-Pleistocene and subsequent adaptation of mussels to different surface water temperature zones may have contributed to shaping the contemporary genetic diversity and deep divergence of the two mitochondrial lineages. In contrast to mtDNA sequences, a clear lineage split between the two mitochondrial lineages was not found in ITS1 sequences, which showed a star-like structure composed of a mixture of southern and northern mitochondrial lineages. Possible reasons for this type of mito-nuclear discordance include stochastic divergence in the coalescent processes of the two molecular markers, or balancing selection under different marine environments. Cryptic speciation cannot be ruled out from these results, and future work using genomic analyses is required to address whether the thermal physiology of these mussels corresponds to the deep divergence of their mitochondrial genes and to test for the existence of morphologically indistinguishable but genetically separate cryptic species.

Original languageEnglish
Article number107037
JournalMolecular Phylogenetics and Evolution
StatePublished - Apr 2021

Bibliographical note

Funding Information:
This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (Nos. 2017R1A2B4011597, 2020R1A2C2005393). This is also supported by a grant from the Marine Biotechnology Program of the Korea Institute of Marine Science and Technology Promotion (KIMST) funded by the Ministry of Oceans and Fisheries (MOF, Korea) (Nos. 20170431, 20170488).

Publisher Copyright:
© 2020 Elsevier Inc.


  • Balancing selection
  • Mito-nuclear discordance
  • Mitochondrial lineage splitting
  • Mytilisepta virgata
  • Northwestern Pacific
  • Phylogeography


Dive into the research topics of 'Phylogeography of Mytilisepta virgata (Mytilidae: Bivalvia) in the northwestern Pacific: Cryptic mitochondrial lineages and mito-nuclear discordance'. Together they form a unique fingerprint.

Cite this