Photothermal PCR chip with plasmonic glass nanopillar arrays

Youngseop Lee, Byoung Hoon Kang, Minhee Kang, Luke P. Lee, Ki Hun Jeong

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

This work presents photothermal polymerase chain reaction (PCR) chip using plasmonic glass nanopillar arrays and a white LED for rapid molecular diagnostic. The plasmonic glass nanopillar arrays consist of glass nanopillar arrays (GNA) and gold nanoislands (AuNIs). The plasmonic nanopillars exhibit highly enhanced light absorption and strongly enhanced local electric field between the AuNIs in a full visible range. The plasmonic nanopillars are fabricated by using solid-state dewetting of silver thin film, reactive ion etching, and thermal evaporation of AuNIs. The photothermal PCR chip show rapid thermal cycles between 60 ℃ and 95 ℃ mirroring annealing and denaturation temperatures of the PCR process (30 cycles, 3 min 30 sec). λ-DNA is successfully amplified by using photothermal PCR chip with the plasmonic nanopillars, a PDMS chamber, and the white LED. This photothermal PCR chip with the plasmonic nanopillars enables ultrafast and portable PCR for molecular diagnostics at point-of-care level.

Original languageEnglish
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Pages1812-1814
Number of pages3
ISBN (Electronic)9781510897571
StatePublished - 2018
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China
Duration: 11 Nov 201815 Nov 2018

Publication series

Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume3

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Country/TerritoryTaiwan, Province of China
CityKaohsiung
Period11/11/1815/11/18

Bibliographical note

Funding Information:
This work was supported by the grant funded by the ministry of Science and ICT of Korea (No. 2018029899, No. 2018018122).

Publisher Copyright:
Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.

Keywords

  • Biochip
  • Photothermal effect
  • Plasmonic nanostructures
  • Rapid PCR

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