Photoacoustic imaging of tumor targeting with biotin conjugated nanostructured phthalocyanine assemblies

Seunghyun Lee, Xingshu Li, Dayoung Lee, Juyoung Yoon, Chulhong Kim

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations


Visualizing biological markers and delivering bioactive agents to living organisms are important to biological research. In recent decades, photoacoustic imaging (PAI) has been significantly improved in the area of molecular imaging, which provides high-resolution volume imaging with high optical absorption contrast. To demonstrate the ability of nanoprobes to target tumors using PAI, we synthesize convertible nanostructured agents with strong photothermal and photoacoustic properties and linked the nanoprobe with biotin to target tumors in small animal model. Interestingly, these nanoprobes allow partial to disassemble in the presence of targeted proteins that switchable photoactivity, thus the nanoprobes provides a fluorescent-cancer imaging with high signal-to-background ratios. The proposed nanoprobe produce a much stronger PA signal compared to the same concentration of methylene blue (MB), which is widely used in clinical study and contrast agent for PAI. The biotin conjugated nanoprobe has high selectivity for biotin receptor positive cancer cells such as A549 (human lung cancer). Then we subsequently examined the PA properties of the nanoprobe that are inherently suitable for in vivo PAI. After injecting of the nanoprobe via intravenous method, we observed the mice's whole body by PA imaging and acquired the PA signal near the cancer. The PA signal increased linearly with time after injection and the fluorescence signal near the cancer was confirmed by fluorescence imaging. The ability to target a specific cancer of the nanoprobe was well verified by PA imaging. This study provides valuable perspective on the advancement of clinical translations and in the design of tumor-targeting phototheranostic agents that could act as new nanomedicines.

Original languageEnglish
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2018
EditorsLihong V. Wang, Alexander A. Oraevsky
ISBN (Electronic)9781510614734
StatePublished - 2018
EventPhotons Plus Ultrasound: Imaging and Sensing 2018 - San Francisco, United States
Duration: 28 Jan 20181 Feb 2018

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferencePhotons Plus Ultrasound: Imaging and Sensing 2018
Country/TerritoryUnited States
CitySan Francisco

Bibliographical note

Funding Information:
This research was supported by grants from Consilience Creative program (IITP-2017-R0346-16-1007 ) of the MSIT (Ministry of Science and ICT) supervised by the IITP (Institute for Information & communications Technology Promotion), the Korea Health Technology R&D Project (HI15C1817) through the KHIDI (Korea Health Industry Development Institute) funded by the Ministry of Health & Welfare and the National Research Foundation of Korea (NRF) grant (No. 2011-0030075) of the MSIP (MSIT and Future Planning). This research was supported by Global PH.

Funding Information:
D Fellowship Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education (NRF-2015H1A2A1031821).

Publisher Copyright:
© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.


  • Biotin conjugated bio marker
  • Photoacoustic imaging
  • Photoacoustic tumor imaging
  • Tumor monitoring
  • Tumor targeting


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