Pericyte-derived extracellular vesicles-mimetic nanovesicles improves peripheral nerve regeneration in mouse models of sciatic nerve transection

Guo Nan Yin, Tae Young Shin, Jiyeon Ock, Min Ji Choi, Anita Limanjaya, Mi Hye Kwon, Fang Yuan Liu, Soon Sun Hong, Ju Hee Kang, Yong Song Gho, Jun Kyu Suh, Ji Kan Ryu

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5 Scopus citations

Abstract

Pericyte-derived extracellular vesicle-mimetic nanovesicles (PC-NVs) play an important role in the improvement of erectile function after cavernous nerve injury. However, the impact of PC-NVs on the peripheral nervous system (PNS), such as the sciatic nerve, is unclear. In this study, PC-NVs were isolated from mouse cavernous pericytes (MCPs). A sciatic nerve transection (SNT) model was established using 8-week-old C57BL/6J mice. The sciatic nerve was harvested 5 and 14 days for immunofluorescence and western blot studies. Function studies were evaluated by performing the rotarod test and walking track analysis. The results demonstrated that PC-NVs could stimulate endothelial cells, increase neuronal cell content, and increase macrophage and Schwann cell presence at the proximal stump rather than the distal stump in the SNT model, thereby improving angiogenesis and nerve regeneration in the early stage of sciatic nerve regeneration. In addition, PC-NVs also increased the expression of neurotrophic factors (brain-derived nerve growth factor, neurotrophin-3 and nerve growth factor) and the activity of the cell survival signaling pathway (PI3K/Akt signaling), and reduced the activity of the JNK signaling pathway. Additionally, after 8 weeks of local application of PC-NVs in SNT model mice, their motor and sensory functions were significantly improved, as assessed by performing the rotarod test and walking track analysis. In conclusion, the present study showed that the significant improvement of neurovascular regeneration in mice following treatment with PC-NVs may provide a favorable strategy for promoting motor and sensory regeneration and functional recovery of the PNS.

Original languageEnglish
Article number5073
JournalInternational Journal of Molecular Medicine
Volume49
Issue number2
DOIs
StatePublished - Feb 2022

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (grant no. 2019R1A2c2002414), the Medical Research center (grant no. NRF‑2021R1A5A2031612), the Joint Grant [composed of the Korean government (Ministry of Science, IcT and Future Planning), the Bio & Medical Technology development Program of the National Research Foundation and the Korean government (MSIT); grant no. 2019069621] and the National Research Foundation of Korea (grant no. 2021R1A2c4002133).

Publisher Copyright:
© 2022 Spandidos Publications. All rights reserved.

Keywords

  • Angiogenesis
  • Endothelial cell
  • Extracellular vesicle
  • Macrophage
  • Nanovesicle
  • Neurotrophic factor
  • Neurovascular regeneration
  • Pericytes
  • Peripheral nervous system
  • Sciatic nerve transection

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