Peptidomimetic small-molecule compounds promoting cardiogenesis of stem cells

Se Woong Oh, Jung Bok Lee, Bora Kim, Sejin Jeon, Min Kyoung Kim, Ki Hoan Nam, Jong Ryul Ha, Mickie Bhatia, Goo Taeg Oh, Dae Yong Kim

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Embryonic stem (ES) cells may be used as an alternative source of functionally intact cardiomyocytes for ischemic heart disease. Several natural and synthetic small molecules have been identified as useful tools for controlling and manipulating stem cell renewal and differentiation. Currently, there is an urgent requirement for novel small molecules that specifically induce differentiation of stem cells into cardiomyocytes. To identify compounds that promote cardiomyogenesis of stem cells, cell-based screening of a peptidomimetic small-molecule library was carried out. A series of β-turn peptidomimetic compounds, including CW209E, increased the expression of α-MHC promoter-driven enhanced green fluorescent protein (EGFP) and ratio of beating embryoid bodies (EBs) without inducing cytotoxicity in mouse embryonic stem cells. CW209E also increased the number of beating EBs in human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). Thus, this chemical compound should be useful for elucidation of the molecular pathway of cardiogenesis and generation of cardiomyocytes ex vivo, which can be further applied for experimental or clinical cell therapy for ischemic heart diseases.

Original languageEnglish
Pages (from-to)1979-1988
Number of pages10
JournalArchives of Pharmacal Research
Volume35
Issue number11
DOIs
StatePublished - Nov 2012

Keywords

  • Cardiogenesis
  • Embryonic stem cells
  • Ischemic heart disease
  • Peptidomimetic compounds

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