Oxysterols induce apoptosis and accumulation of cell cycle at G2/M phase in the human monocytic THP-1 cell line

Hee Kyoung Lim; Hee Kyoung Kang; Eun Sook Yoo; Bum Joon Kim; Youn Woo Kim; Moonjae Cho; Jae Hwa Lee; Yun Sil Lee; Myung Hee Chung; Jin Won Hyun

doi:10.1016/S0024-3205(02)02377-9

Oxysterols induce apoptosis and accumulation of cell cycle at G₂/M phase in the human monocytic THP-1 cell line

Hee Kyoung Lim, Hee Kyoung Kang, Eun Sook Yoo, Bum Joon Kim, Youn Woo Kim, Moonjae Cho, Jae Hwa Lee, Yun Sil Lee, Myung Hee Chung, Jin Won Hyun

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

The cytotoxic activity of oxysterols, 7β-hydroxycholesterol (7β-OHC) and 25-hydroxycholesterol (25-OHC), has been evaluated using various leukemia cell lines. Among the tested cell lines, both oxysterols showed the highest cytotoxicity to THP-1, human monocytic leukemia cell line. These oxysterols induced apoptosis through down-regulation of Bcl-2 expression and activation of caspases. Also, the oxysterols showed the accumulation at G₂/M phase of cell cycle through down-regulation of cyclin B1 expression. Taken together, these results indicated that both 7β-OHC and 25-OHC inhibited the proliferation of THP-1 cells through apoptosis and cell cycle accumulation at G₂/M phase.

Original language	English
Pages (from-to)	1389-1399
Number of pages	11
Journal	Life Sciences
Volume	72
Issue number	12
DOIs	https://doi.org/10.1016/S0024-3205(02)02377-9
State	Published - 7 Feb 2003

Bibliographical note

Funding Information:
This work was supported by grants from the Cheju National University Medical Research Fund (2002) and the Ministry of Science and Technology of Korea through the National Research Laboratory Program for Free Radicals.

Keywords

Apoptosis
Cell cycle
Cytotoxicity
Oxysterol

Access to Document

10.1016/S0024-3205(02)02377-9

Cite this

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title = "Oxysterols induce apoptosis and accumulation of cell cycle at G2/M phase in the human monocytic THP-1 cell line",

abstract = "The cytotoxic activity of oxysterols, 7β-hydroxycholesterol (7β-OHC) and 25-hydroxycholesterol (25-OHC), has been evaluated using various leukemia cell lines. Among the tested cell lines, both oxysterols showed the highest cytotoxicity to THP-1, human monocytic leukemia cell line. These oxysterols induced apoptosis through down-regulation of Bcl-2 expression and activation of caspases. Also, the oxysterols showed the accumulation at G2/M phase of cell cycle through down-regulation of cyclin B1 expression. Taken together, these results indicated that both 7β-OHC and 25-OHC inhibited the proliferation of THP-1 cells through apoptosis and cell cycle accumulation at G2/M phase.",

keywords = "Apoptosis, Cell cycle, Cytotoxicity, Oxysterol",

author = "Lim, {Hee Kyoung} and Kang, {Hee Kyoung} and Yoo, {Eun Sook} and Kim, {Bum Joon} and Kim, {Youn Woo} and Moonjae Cho and Lee, {Jae Hwa} and Lee, {Yun Sil} and Chung, {Myung Hee} and Hyun, {Jin Won}",

note = "Funding Information: This work was supported by grants from the Cheju National University Medical Research Fund (2002) and the Ministry of Science and Technology of Korea through the National Research Laboratory Program for Free Radicals.",

year = "2003",

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doi = "10.1016/S0024-3205(02)02377-9",

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T1 - Oxysterols induce apoptosis and accumulation of cell cycle at G2/M phase in the human monocytic THP-1 cell line

AU - Lim, Hee Kyoung

AU - Kang, Hee Kyoung

AU - Yoo, Eun Sook

AU - Kim, Bum Joon

AU - Kim, Youn Woo

AU - Cho, Moonjae

AU - Lee, Jae Hwa

AU - Lee, Yun Sil

AU - Chung, Myung Hee

AU - Hyun, Jin Won

N1 - Funding Information: This work was supported by grants from the Cheju National University Medical Research Fund (2002) and the Ministry of Science and Technology of Korea through the National Research Laboratory Program for Free Radicals.

PY - 2003/2/7

Y1 - 2003/2/7

N2 - The cytotoxic activity of oxysterols, 7β-hydroxycholesterol (7β-OHC) and 25-hydroxycholesterol (25-OHC), has been evaluated using various leukemia cell lines. Among the tested cell lines, both oxysterols showed the highest cytotoxicity to THP-1, human monocytic leukemia cell line. These oxysterols induced apoptosis through down-regulation of Bcl-2 expression and activation of caspases. Also, the oxysterols showed the accumulation at G2/M phase of cell cycle through down-regulation of cyclin B1 expression. Taken together, these results indicated that both 7β-OHC and 25-OHC inhibited the proliferation of THP-1 cells through apoptosis and cell cycle accumulation at G2/M phase.

AB - The cytotoxic activity of oxysterols, 7β-hydroxycholesterol (7β-OHC) and 25-hydroxycholesterol (25-OHC), has been evaluated using various leukemia cell lines. Among the tested cell lines, both oxysterols showed the highest cytotoxicity to THP-1, human monocytic leukemia cell line. These oxysterols induced apoptosis through down-regulation of Bcl-2 expression and activation of caspases. Also, the oxysterols showed the accumulation at G2/M phase of cell cycle through down-regulation of cyclin B1 expression. Taken together, these results indicated that both 7β-OHC and 25-OHC inhibited the proliferation of THP-1 cells through apoptosis and cell cycle accumulation at G2/M phase.

KW - Apoptosis

KW - Cell cycle

KW - Cytotoxicity

KW - Oxysterol

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