Abstract
The cytotoxic activity of oxysterols, 7β-hydroxycholesterol (7β-OHC) and 25-hydroxycholesterol (25-OHC), has been evaluated using various leukemia cell lines. Among the tested cell lines, both oxysterols showed the highest cytotoxicity to THP-1, human monocytic leukemia cell line. These oxysterols induced apoptosis through down-regulation of Bcl-2 expression and activation of caspases. Also, the oxysterols showed the accumulation at G2/M phase of cell cycle through down-regulation of cyclin B1 expression. Taken together, these results indicated that both 7β-OHC and 25-OHC inhibited the proliferation of THP-1 cells through apoptosis and cell cycle accumulation at G2/M phase.
Original language | English |
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Pages (from-to) | 1389-1399 |
Number of pages | 11 |
Journal | Life Sciences |
Volume | 72 |
Issue number | 12 |
DOIs | |
State | Published - 7 Feb 2003 |
Keywords
- Apoptosis
- Cell cycle
- Cytotoxicity
- Oxysterol