Oxysterols induce apoptosis and accumulation of cell cycle at G2/M phase in the human monocytic THP-1 cell line

Hee Kyoung Lim, Hee Kyoung Kang, Eun Sook Yoo, Bum Joon Kim, Youn Woo Kim, Moonjae Cho, Jae Hwa Lee, Yun Sil Lee, Myung Hee Chung, Jin Won Hyun

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

The cytotoxic activity of oxysterols, 7β-hydroxycholesterol (7β-OHC) and 25-hydroxycholesterol (25-OHC), has been evaluated using various leukemia cell lines. Among the tested cell lines, both oxysterols showed the highest cytotoxicity to THP-1, human monocytic leukemia cell line. These oxysterols induced apoptosis through down-regulation of Bcl-2 expression and activation of caspases. Also, the oxysterols showed the accumulation at G2/M phase of cell cycle through down-regulation of cyclin B1 expression. Taken together, these results indicated that both 7β-OHC and 25-OHC inhibited the proliferation of THP-1 cells through apoptosis and cell cycle accumulation at G2/M phase.

Original languageEnglish
Pages (from-to)1389-1399
Number of pages11
JournalLife Sciences
Volume72
Issue number12
DOIs
StatePublished - 7 Feb 2003

Keywords

  • Apoptosis
  • Cell cycle
  • Cytotoxicity
  • Oxysterol

Fingerprint

Dive into the research topics of 'Oxysterols induce apoptosis and accumulation of cell cycle at G2/M phase in the human monocytic THP-1 cell line'. Together they form a unique fingerprint.

Cite this