Oxidized low-density lipoprotein- and lysophostidylcholine-induced Ca2+ mobilization in human endothelial cells

Moon Young Kim, Guo Hua Liang, Ji Aee Kim, Soo Seung Choi, Shinku Choi, Suk Hyo Suh

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11 Scopus citations

Abstract

The effects of oxidized low-density lipoprotein (OxLDL) and its major lipid constituent lysophosphatidylcholine (LPC) on Ca2+ entry were investigated in cultured human umbilical endothelial cells (HUVECs) using fura-2 fluorescence and patch-clamp methods. OxLDL or LPC increased intracellular Ca2+ concentration ([Ca2+]i), and the increase of [Ca2+]i by OxLDL or by LPC was inhibited by La3+ or heparin. LPC failed to increase [Ca2+]i in the presence of an antioxidant tempol. In addition, store-operated Ca2+ entry (SOC), which was evoked by intracellular Ca2+ store depletion in Ca2+-free solution using the sarcoplasmic reticulum Ca2+ pump blocker, 2, 5-di-t-butyl-1, 4-benzohydroquinone (BHQY), was further enhanced by OxLDL or by LPC. Increased SOC by OxLDL or by LPC was inhibited by U73122. In voltage-clamped cells, OxLDL or LPC increased [Ca2+]i and simultaneously activated non-selective cation (NSC) currents. LPC-induced NSC currents were inhibited by 2-APB, La3+ or U73122, and NSC currents were not activated by LPC in the presence of tempol. Furthermore, in voltage-clamped HUVECs, OxLDL enhanced SOC and evoked outward currents simultaneously. Clampinig intracellular Ca2+ to 1 υM activated large-conductance Ca2+-activated K+ (BKCa) clirrent spontaneously, and this activated BKCa current was further enhanced bv OxLDL or by LPC. From these results, we concluded that OxLDL or its main component LPC activates Ca2+-permeable Ca2+-activated NSC current and BKCa current simultaneously, thereby increasing SOC.

Original languageEnglish
Pages (from-to)27-32
Number of pages6
JournalKorean Journal of Physiology and Pharmacology
Volume13
Issue number1
DOIs
StatePublished - Feb 2009

Keywords

  • Endothelial cell
  • K channel
  • Large conductance Ca -activated
  • Nonselective cation current
  • Oxidized LDL
  • Store-operated Ca entry

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