Oxidation of fatty acid may be enhanced by a combination of pomegranate fruit phytochemicals and acetic acid in HepG2 cells

Ji Yeon Kim, Elly Ok, You Jin Kim, Kyoung Sook Choi, Oran Kwon

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

We investigated whether the combination of phytochemicals and acetic acid in the form of fruit vinegar provides an additive effect on changes of mRNA levels related to fatty acid oxidation in human hepatocyte (HepG2). Among the seven fruit vinegars (Rubuscoreanus, Opuntia, blueberry, cherry, red ginseng, mulberry, and pomegranate) studied, treatment of HepG2 with pomegranate vinegar (PV) at concentrations containing 1 mM acetic acid showed the highest in vitro potentiating effect on the mRNA expression levels of peroxisome proliferator-activated receptor α, carnitinepalmitoyl transferase-1, and acyl-CoA oxidase compared to the control group (P < 0.05). Reversed-phase liquid chromatography in combination with quadrupole time-of-flight mass spectrometry analysis revealed four potential compounds (punicalagin B, ellagic acid, and two unidentified compounds) responsible for altered gene expression in HepG2 cells treated with PV as compared with the others. Further investigations are warranted to determine if drinking PV beverages may help to maintain a healthy body weight in overweight subjects.

Original languageEnglish
Pages (from-to)153-159
Number of pages7
JournalNutrition Research and Practice
Volume7
Issue number3
DOIs
StatePublished - Jun 2013

Keywords

  • Acetic acid
  • Fatty acid oxidation
  • HepG2 cells
  • Pomegranate
  • Vinegar beverage

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