Overexpression of Neurospora crassa OR74A glutamate decarboxylase in Escherichia coli for efficient GABA production

Tam Dinh Le Vo, Ji Seun Ko, Seung Hwan Lee, Si Jae Park, Soon Ho Hong

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

This study investigated the effect of glutamate decarboxylase from Neurospora crassa OR74A on GABA production in Escherichia coli. GABA is one of the inhibitory neurotransmitters in the mammalian central nervous system, and can be used as a precursor of promising biopolymer Nylon 4. E. coli that overexpressed N. crassa glutamate decarboxylase was cultured at various pH levels and temperatures to determine optimum conditions for GABA production. When the recombinant E. coli strain was cultured at 30 C and pH 3, a final GABA concentration of 5.26 g/L was obtained from 10 g/L of monosodium glutamate (MSG), corresponding to a GABA yield of 86.23%.

Original languageEnglish
Pages (from-to)1062-1066
Number of pages5
JournalBiotechnology and Bioprocess Engineering
Volume18
Issue number6
DOIs
StatePublished - Nov 2013

Keywords

  • Escherichia coli
  • gamma-aminobutyric acid
  • glutamate decarboxylase
  • Neurospora crassa

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