Overexpression of Neurospora crassa OR74A glutamate decarboxylase in Escherichia coli for efficient GABA production

Tam Dinh Le Vo; Ji Seun Ko; Seung Hwan Lee; Si Jae Park; Soon Ho Hong

doi:10.1007/s12257-013-0282-8

Overexpression of Neurospora crassa OR74A glutamate decarboxylase in Escherichia coli for efficient GABA production

Tam Dinh Le Vo, Ji Seun Ko, Seung Hwan Lee, Si Jae Park, Soon Ho Hong

Department of Chemical Engineering & Materials Science

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

This study investigated the effect of glutamate decarboxylase from Neurospora crassa OR74A on GABA production in Escherichia coli. GABA is one of the inhibitory neurotransmitters in the mammalian central nervous system, and can be used as a precursor of promising biopolymer Nylon 4. E. coli that overexpressed N. crassa glutamate decarboxylase was cultured at various pH levels and temperatures to determine optimum conditions for GABA production. When the recombinant E. coli strain was cultured at 30 C and pH 3, a final GABA concentration of 5.26 g/L was obtained from 10 g/L of monosodium glutamate (MSG), corresponding to a GABA yield of 86.23%.

Original language	English
Pages (from-to)	1062-1066
Number of pages	5
Journal	Biotechnology and Bioprocess Engineering
Volume	18
Issue number	6
DOIs	https://doi.org/10.1007/s12257-013-0282-8
State	Published - Nov 2013

Bibliographical note

Funding Information:
This work was supported by a grant from the Next-Generation BioGreen 21 Program (SSAC, grant number: PJ00954904), Rural Development Administration, Republic of Korea.

Keywords

Escherichia coli
Neurospora crassa
gamma-aminobutyric acid
glutamate decarboxylase

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10.1007/s12257-013-0282-8

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title = "Overexpression of Neurospora crassa OR74A glutamate decarboxylase in Escherichia coli for efficient GABA production",

abstract = "This study investigated the effect of glutamate decarboxylase from Neurospora crassa OR74A on GABA production in Escherichia coli. GABA is one of the inhibitory neurotransmitters in the mammalian central nervous system, and can be used as a precursor of promising biopolymer Nylon 4. E. coli that overexpressed N. crassa glutamate decarboxylase was cultured at various pH levels and temperatures to determine optimum conditions for GABA production. When the recombinant E. coli strain was cultured at 30 C and pH 3, a final GABA concentration of 5.26 g/L was obtained from 10 g/L of monosodium glutamate (MSG), corresponding to a GABA yield of 86.23%.",

keywords = "Escherichia coli, Neurospora crassa, gamma-aminobutyric acid, glutamate decarboxylase",

author = "{Le Vo}, {Tam Dinh} and Ko, {Ji Seun} and Lee, {Seung Hwan} and Park, {Si Jae} and Hong, {Soon Ho}",

note = "Funding Information: This work was supported by a grant from the Next-Generation BioGreen 21 Program (SSAC, grant number: PJ00954904), Rural Development Administration, Republic of Korea.",

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doi = "10.1007/s12257-013-0282-8",

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T1 - Overexpression of Neurospora crassa OR74A glutamate decarboxylase in Escherichia coli for efficient GABA production

AU - Le Vo, Tam Dinh

AU - Ko, Ji Seun

AU - Lee, Seung Hwan

AU - Park, Si Jae

AU - Hong, Soon Ho

N1 - Funding Information: This work was supported by a grant from the Next-Generation BioGreen 21 Program (SSAC, grant number: PJ00954904), Rural Development Administration, Republic of Korea.

PY - 2013/11

Y1 - 2013/11

N2 - This study investigated the effect of glutamate decarboxylase from Neurospora crassa OR74A on GABA production in Escherichia coli. GABA is one of the inhibitory neurotransmitters in the mammalian central nervous system, and can be used as a precursor of promising biopolymer Nylon 4. E. coli that overexpressed N. crassa glutamate decarboxylase was cultured at various pH levels and temperatures to determine optimum conditions for GABA production. When the recombinant E. coli strain was cultured at 30 C and pH 3, a final GABA concentration of 5.26 g/L was obtained from 10 g/L of monosodium glutamate (MSG), corresponding to a GABA yield of 86.23%.

AB - This study investigated the effect of glutamate decarboxylase from Neurospora crassa OR74A on GABA production in Escherichia coli. GABA is one of the inhibitory neurotransmitters in the mammalian central nervous system, and can be used as a precursor of promising biopolymer Nylon 4. E. coli that overexpressed N. crassa glutamate decarboxylase was cultured at various pH levels and temperatures to determine optimum conditions for GABA production. When the recombinant E. coli strain was cultured at 30 C and pH 3, a final GABA concentration of 5.26 g/L was obtained from 10 g/L of monosodium glutamate (MSG), corresponding to a GABA yield of 86.23%.

KW - Escherichia coli

KW - Neurospora crassa

KW - gamma-aminobutyric acid

KW - glutamate decarboxylase

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Overexpression of Neurospora crassa OR74A glutamate decarboxylase in Escherichia coli for efficient GABA production

Abstract

Bibliographical note

Keywords

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