Overexpression, crystallization and preliminary X-ray crystallographic analysis of hypothetical protein SAV0479 from Staphylococcus aureus Mu50

Chinar Pathak; Sun Bok Jang; Hookang Im; Hye Jin Yoon; Bong Jin Lee

doi:10.1107/S1744309113003783

Overexpression, crystallization and preliminary X-ray crystallographic analysis of hypothetical protein SAV0479 from Staphylococcus aureus Mu50

Chinar Pathak
, Sun Bok Jang
, Hookang Im
, Hye Jin Yoon
, Bong Jin Lee

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

SAV0479, a hypothetical protein from the Mu50 strain of methicillin- and vancomycin-resistant Staphylococcus aureus, was selected for structure and function determination as part of a structural genomics project. Here, the cloning, overexpression, purification and crystallization of SAV0479 are reported. Crystals were obtained by the hanging-drop vapour-diffusion method and diffraction data were collected to a resolution of 2.8 Å. The crystals belonged to space group P3121, with unit-cell parameters a = b = 81.48, c = 82.53 Å. Three monomers of SAV0479 are present in each asymmetric unit.

Original language	English
Pages (from-to)	405-407
Number of pages	3
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	69
Issue number	4
DOIs	https://doi.org/10.1107/S1744309113003783
State	Published - Apr 2013

Keywords

hypothetical protein SAV0479
methicillin- and vancomycin-resistant Staphylococcus aureus

Access to Document

10.1107/S1744309113003783

Cite this

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title = "Overexpression, crystallization and preliminary X-ray crystallographic analysis of hypothetical protein SAV0479 from Staphylococcus aureus Mu50",

abstract = "SAV0479, a hypothetical protein from the Mu50 strain of methicillin- and vancomycin-resistant Staphylococcus aureus, was selected for structure and function determination as part of a structural genomics project. Here, the cloning, overexpression, purification and crystallization of SAV0479 are reported. Crystals were obtained by the hanging-drop vapour-diffusion method and diffraction data were collected to a resolution of 2.8 {\AA}. The crystals belonged to space group P3121, with unit-cell parameters a = b = 81.48, c = 82.53 {\AA}. Three monomers of SAV0479 are present in each asymmetric unit.",

keywords = "hypothetical protein SAV0479, methicillin- and vancomycin-resistant Staphylococcus aureus",

author = "Chinar Pathak and Jang, \{Sun Bok\} and Hookang Im and Yoon, \{Hye Jin\} and Lee, \{Bong Jin\}",

year = "2013",

month = apr,

doi = "10.1107/S1744309113003783",

language = "English",

volume = "69",

pages = "405--407",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

issn = "1744-3091",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "4",

}

Overexpression, crystallization and preliminary X-ray crystallographic analysis of hypothetical protein SAV0479 from Staphylococcus aureus Mu50. / Pathak, Chinar; Jang, Sun Bok; Im, Hookang et al.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 69, No. 4, 04.2013, p. 405-407.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Overexpression, crystallization and preliminary X-ray crystallographic analysis of hypothetical protein SAV0479 from Staphylococcus aureus Mu50

AU - Pathak, Chinar

AU - Jang, Sun Bok

AU - Im, Hookang

AU - Yoon, Hye Jin

AU - Lee, Bong Jin

PY - 2013/4

Y1 - 2013/4

N2 - SAV0479, a hypothetical protein from the Mu50 strain of methicillin- and vancomycin-resistant Staphylococcus aureus, was selected for structure and function determination as part of a structural genomics project. Here, the cloning, overexpression, purification and crystallization of SAV0479 are reported. Crystals were obtained by the hanging-drop vapour-diffusion method and diffraction data were collected to a resolution of 2.8 Å. The crystals belonged to space group P3121, with unit-cell parameters a = b = 81.48, c = 82.53 Å. Three monomers of SAV0479 are present in each asymmetric unit.

AB - SAV0479, a hypothetical protein from the Mu50 strain of methicillin- and vancomycin-resistant Staphylococcus aureus, was selected for structure and function determination as part of a structural genomics project. Here, the cloning, overexpression, purification and crystallization of SAV0479 are reported. Crystals were obtained by the hanging-drop vapour-diffusion method and diffraction data were collected to a resolution of 2.8 Å. The crystals belonged to space group P3121, with unit-cell parameters a = b = 81.48, c = 82.53 Å. Three monomers of SAV0479 are present in each asymmetric unit.

KW - hypothetical protein SAV0479

KW - methicillin- and vancomycin-resistant Staphylococcus aureus

UR - https://www.scopus.com/pages/publications/84875842692

U2 - 10.1107/S1744309113003783

DO - 10.1107/S1744309113003783

M3 - Article

C2 - 23545646

AN - SCOPUS:84875842692

SN - 1744-3091

VL - 69

SP - 405

EP - 407

JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

IS - 4

ER -

Overexpression, crystallization and preliminary X-ray crystallographic analysis of hypothetical protein SAV0479 from Staphylococcus aureus Mu50

Abstract

Keywords

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