TY - JOUR
T1 - Overexpression, crystallization and preliminary X-ray crystallographic analysis of hypothetical protein SAV0479 from Staphylococcus aureus Mu50
AU - Pathak, Chinar
AU - Jang, Sun Bok
AU - Im, Hookang
AU - Yoon, Hye Jin
AU - Lee, Bong Jin
PY - 2013/4
Y1 - 2013/4
N2 - SAV0479, a hypothetical protein from the Mu50 strain of methicillin- and vancomycin-resistant Staphylococcus aureus, was selected for structure and function determination as part of a structural genomics project. Here, the cloning, overexpression, purification and crystallization of SAV0479 are reported. Crystals were obtained by the hanging-drop vapour-diffusion method and diffraction data were collected to a resolution of 2.8 Å. The crystals belonged to space group P3121, with unit-cell parameters a = b = 81.48, c = 82.53 Å. Three monomers of SAV0479 are present in each asymmetric unit.
AB - SAV0479, a hypothetical protein from the Mu50 strain of methicillin- and vancomycin-resistant Staphylococcus aureus, was selected for structure and function determination as part of a structural genomics project. Here, the cloning, overexpression, purification and crystallization of SAV0479 are reported. Crystals were obtained by the hanging-drop vapour-diffusion method and diffraction data were collected to a resolution of 2.8 Å. The crystals belonged to space group P3121, with unit-cell parameters a = b = 81.48, c = 82.53 Å. Three monomers of SAV0479 are present in each asymmetric unit.
KW - hypothetical protein SAV0479
KW - methicillin- and vancomycin-resistant Staphylococcus aureus
UR - http://www.scopus.com/inward/record.url?scp=84875842692&partnerID=8YFLogxK
U2 - 10.1107/S1744309113003783
DO - 10.1107/S1744309113003783
M3 - Article
C2 - 23545646
AN - SCOPUS:84875842692
SN - 1744-3091
VL - 69
SP - 405
EP - 407
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
IS - 4
ER -