Over-Expression of a RhoA-Specific Guanine Nucleotide Exchange Factor, p190RhoGEF, in Mouse Dendritic Cells Negatively Regulates Cellular Responses to Bacterial Lipopolysaccharide

Hee Jung Seul; Yu Ri Ahn; Hyeon Myeong Song; Yun Jung Ha; Jong Ran Lee

doi:10.1007/s10059-012-0055-9

Over-Expression of a RhoA-Specific Guanine Nucleotide Exchange Factor, p190RhoGEF, in Mouse Dendritic Cells Negatively Regulates Cellular Responses to Bacterial Lipopolysaccharide

Hee Jung Seul, Yu Ri Ahn, Hyeon Myeong Song, Yun Jung Ha, Jong Ran Lee

Department of Life Science

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

We studied the role of a RhoA-specific guanine nucleotide exchange factor (p190RhoGEF) in dendritic cells (DCs), using transgenic (TG) mice that over-express a full gene of p190RhoGEF under the control of an invariant chain promoter. TG mice lacked localization of activated DCs to the T cell zone in the spleen and had reduced serum levels of IL-6 in response to lipopolysaccharide (LPS) injection. DCs from these mice also showed reduced surface expression of CD86, CD40, and CD205, but not MHCII, as well as a reduced capability to uptake antigen. Moreover, chemokinedriven migration and secretion of IL-6, but not of IL-12, were impaired after LPS-stimulation of TG DCs. Collectively, these results suggest that over-expressing p190RhoGEF negatively regulates conventional DC function in response to bacterial LPS infection.

Original language	English
Pages (from-to)	159-164
Number of pages	6
Journal	Molecules and Cells
Volume	34
Issue number	2
DOIs	https://doi.org/10.1007/s10059-012-0055-9
State	Published - Aug 2012

Bibliographical note

Funding Information:
We thank Drs. W. Moolenaar and D. Mathis for providing p190RhoGEF plasmid and pDOI expression vector, respectively. This study was supported by a grant of the Korea Healthcare technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (A090743) and grants (2009-0067361, 2012-0000952) from the National Research Foundation of Korea funded by the Korean government. H.J.S., Y.R.A., H.M.S., and Y.J.H. were supported in part by the second stage of the Brain Korea 21 Program of the Korea Ministry of Education.

Keywords

Dendritic cells
Lipopolysaccharides
P190RhoGEF

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title = "Over-Expression of a RhoA-Specific Guanine Nucleotide Exchange Factor, p190RhoGEF, in Mouse Dendritic Cells Negatively Regulates Cellular Responses to Bacterial Lipopolysaccharide",

abstract = "We studied the role of a RhoA-specific guanine nucleotide exchange factor (p190RhoGEF) in dendritic cells (DCs), using transgenic (TG) mice that over-express a full gene of p190RhoGEF under the control of an invariant chain promoter. TG mice lacked localization of activated DCs to the T cell zone in the spleen and had reduced serum levels of IL-6 in response to lipopolysaccharide (LPS) injection. DCs from these mice also showed reduced surface expression of CD86, CD40, and CD205, but not MHCII, as well as a reduced capability to uptake antigen. Moreover, chemokinedriven migration and secretion of IL-6, but not of IL-12, were impaired after LPS-stimulation of TG DCs. Collectively, these results suggest that over-expressing p190RhoGEF negatively regulates conventional DC function in response to bacterial LPS infection.",

keywords = "Dendritic cells, Lipopolysaccharides, P190RhoGEF",

author = "Seul, {Hee Jung} and Ahn, {Yu Ri} and Song, {Hyeon Myeong} and Ha, {Yun Jung} and Lee, {Jong Ran}",

note = "Funding Information: We thank Drs. W. Moolenaar and D. Mathis for providing p190RhoGEF plasmid and pDOI expression vector, respectively. This study was supported by a grant of the Korea Healthcare technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (A090743) and grants (2009-0067361, 2012-0000952) from the National Research Foundation of Korea funded by the Korean government. H.J.S., Y.R.A., H.M.S., and Y.J.H. were supported in part by the second stage of the Brain Korea 21 Program of the Korea Ministry of Education.",

year = "2012",

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doi = "10.1007/s10059-012-0055-9",

language = "English",

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pages = "159--164",

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AU - Seul, Hee Jung

AU - Ahn, Yu Ri

AU - Song, Hyeon Myeong

AU - Ha, Yun Jung

AU - Lee, Jong Ran

N1 - Funding Information: We thank Drs. W. Moolenaar and D. Mathis for providing p190RhoGEF plasmid and pDOI expression vector, respectively. This study was supported by a grant of the Korea Healthcare technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (A090743) and grants (2009-0067361, 2012-0000952) from the National Research Foundation of Korea funded by the Korean government. H.J.S., Y.R.A., H.M.S., and Y.J.H. were supported in part by the second stage of the Brain Korea 21 Program of the Korea Ministry of Education.

PY - 2012/8

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N2 - We studied the role of a RhoA-specific guanine nucleotide exchange factor (p190RhoGEF) in dendritic cells (DCs), using transgenic (TG) mice that over-express a full gene of p190RhoGEF under the control of an invariant chain promoter. TG mice lacked localization of activated DCs to the T cell zone in the spleen and had reduced serum levels of IL-6 in response to lipopolysaccharide (LPS) injection. DCs from these mice also showed reduced surface expression of CD86, CD40, and CD205, but not MHCII, as well as a reduced capability to uptake antigen. Moreover, chemokinedriven migration and secretion of IL-6, but not of IL-12, were impaired after LPS-stimulation of TG DCs. Collectively, these results suggest that over-expressing p190RhoGEF negatively regulates conventional DC function in response to bacterial LPS infection.

AB - We studied the role of a RhoA-specific guanine nucleotide exchange factor (p190RhoGEF) in dendritic cells (DCs), using transgenic (TG) mice that over-express a full gene of p190RhoGEF under the control of an invariant chain promoter. TG mice lacked localization of activated DCs to the T cell zone in the spleen and had reduced serum levels of IL-6 in response to lipopolysaccharide (LPS) injection. DCs from these mice also showed reduced surface expression of CD86, CD40, and CD205, but not MHCII, as well as a reduced capability to uptake antigen. Moreover, chemokinedriven migration and secretion of IL-6, but not of IL-12, were impaired after LPS-stimulation of TG DCs. Collectively, these results suggest that over-expressing p190RhoGEF negatively regulates conventional DC function in response to bacterial LPS infection.

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Over-Expression of a RhoA-Specific Guanine Nucleotide Exchange Factor, p190RhoGEF, in Mouse Dendritic Cells Negatively Regulates Cellular Responses to Bacterial Lipopolysaccharide

Abstract

Bibliographical note

Keywords

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