Outer membrane vesicles derived from Escherichia coli regulate neutrophil migration by induction of endothelial IL-8

Jaewook Lee, Yae Jin Yoon, Ji Hyun Kim, Nhung Thi Hong Dinh, Gyeongyun Go, Sookil Tae, Kyong Su Park, Hyun Taek Park, Changjin Lee, Tae Young Roh, Dolores Di Vizio, Yong Song Gho

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

Outer membrane vesicles (OMVs) are spherical proteolipid nanostructures that are constitutively released by Gram-negative bacteria including Escherichia coli. Although it has been shown that administration of E. coli OMVs stimulates a strong pulmonary inflammatory response with infiltration of neutrophils into the lungs in vivo, the mechanism of E. coli OMV-mediated neutrophil recruitment is poorly characterized. In this study, we observed significant infiltration of neutrophils into the mouse lung tissues in vivo, with increased expression of the neutrophil chemoattractant CXCL1, a murine functional homolog of human IL-8, on intraperitoneal administration of E. coli OMVs. In addition, OMVs and CD31-positive endothelial cells colocalized in the mouse lungs. Moreover, in vitro results showed that E. coli OMVs significantly increased IL-8 release from human microvascular endothelial cells and toll-like receptor (TLR)4 was found to be the main component for recognizing E. coli OMVs among human endothelial cell-associated TLRs. Furthermore, the transmigration of neutrophils was suppressed in the lung tissues obtained from TLR4 knockout mice treated with E. coli OMVs. Taken together, our data demonstrated that E. coli OMVs potently recruit neutrophils into the lung via the release of IL-8/CXCL1 from endothelial cells in TLR4- and NF-κB-dependent manners.

Original languageEnglish
Article number2268
JournalFrontiers in Microbiology
Volume9
Issue numberOCT
DOIs
StatePublished - 11 Oct 2018

Bibliographical note

Funding Information:
This research was supported in part by grant No. 804600 from the U.S. Environmental Protection Agency to the University of Minnesota

Publisher Copyright:
© 2018 Lee, Yoon, Kim, Dinh, Go, Tae, Park, Park, Lee, Roh, Di Vizio and Gho.

Keywords

  • Exosomes
  • Extracellular vesicles
  • IL-8
  • Neutrophil
  • NF-κB
  • Outer membrane vesicles
  • Pulmonary inflammation
  • Toll-like receptor 4

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