Ottogi Inhibits Wnt/β-catenin Signaling by Regulating Cell Membrane Trafficking of Frizzled8

Hyun Taek Kim; Mi Sun Lee; Yun Mi Jeong; Hyunju Ro; Dong Il Kim; Yong Hwan Shin; Ji Eun Kim; Kyu Seok Hwang; Jung Hwa Choi; Minjin Bahn; Jeong Ju Lee; Sang H. Lee; Young Ki Bae; Jin Soo Lee; Joong Kook Choi; Nam Soon Kim; Chang Yeol Yeo; Cheol Hee Kim

doi:10.1038/s41598-017-13429-6

Ottogi Inhibits Wnt/β-catenin Signaling by Regulating Cell Membrane Trafficking of Frizzled8

Hyun Taek Kim, Mi Sun Lee, Yun Mi Jeong, Hyunju Ro, Dong Il Kim, Yong Hwan Shin, Ji Eun Kim, Kyu Seok Hwang, Jung Hwa Choi, Minjin Bahn, Jeong Ju Lee, Sang H. Lee, Young Ki Bae, Jin Soo Lee, Joong Kook Choi, Nam Soon Kim, Chang Yeol Yeo, Cheol Hee Kim

Life Sciences

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Wnt signaling controls critical developmental processes including tissue/body patterning. Here we report the identification of a novel regulator of Wnt signaling, OTTOGI (OTG), isolated from a large-scale expression screening of human cDNAs in zebrafish embryos. Overexpression of OTG in zebrafish embryos caused dorso-Anteriorized phenotype, inhibited the expression of Wnt target genes, and prevented nuclear accumulation of β-catenin. Conversely, knockdown of zebrafish otg using specific antisense morpholino promoted nuclear accumulation of β-catenin and caused ventralization. However, OTG failed to rescue headless-like phenotype induced by inhibition of GSK-3β activity, suggesting that OTG acts upstream of GSK-3β. OTG bound specifically to Frizzled8 (Fz8) receptor and caused retention of Fz8 in the endoplasmic reticulum possibly by preventing N-linked glycosylation of Fz8. Taken together, our data indicate that OTG functions as a novel negative regulator of Wnt signaling during development by the modulation of cell surface expression of Fz receptor.

Original language	English
Article number	13278
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-13429-6
State	Published - 1 Dec 2017

Access to Document

10.1038/s41598-017-13429-6

Cite this

Kim, H. T., Lee, M. S., Jeong, Y. M., Ro, H., Kim, D. I., Shin, Y. H., Kim, J. E., Hwang, K. S., Choi, J. H., Bahn, M., Lee, J. J., Lee, S. H., Bae, Y. K., Lee, J. S., Choi, J. K., Kim, N. S., Yeo, C. Y., & Kim, C. H. (2017). Ottogi Inhibits Wnt/β-catenin Signaling by Regulating Cell Membrane Trafficking of Frizzled8. Scientific Reports, 7(1), [13278]. https://doi.org/10.1038/s41598-017-13429-6

@article{8bfab877ea0346139c5212ec4e6e9070,

title = "Ottogi Inhibits Wnt/β-catenin Signaling by Regulating Cell Membrane Trafficking of Frizzled8",

abstract = "Wnt signaling controls critical developmental processes including tissue/body patterning. Here we report the identification of a novel regulator of Wnt signaling, OTTOGI (OTG), isolated from a large-scale expression screening of human cDNAs in zebrafish embryos. Overexpression of OTG in zebrafish embryos caused dorso-Anteriorized phenotype, inhibited the expression of Wnt target genes, and prevented nuclear accumulation of β-catenin. Conversely, knockdown of zebrafish otg using specific antisense morpholino promoted nuclear accumulation of β-catenin and caused ventralization. However, OTG failed to rescue headless-like phenotype induced by inhibition of GSK-3β activity, suggesting that OTG acts upstream of GSK-3β. OTG bound specifically to Frizzled8 (Fz8) receptor and caused retention of Fz8 in the endoplasmic reticulum possibly by preventing N-linked glycosylation of Fz8. Taken together, our data indicate that OTG functions as a novel negative regulator of Wnt signaling during development by the modulation of cell surface expression of Fz receptor.",

author = "Kim, {Hyun Taek} and Lee, {Mi Sun} and Jeong, {Yun Mi} and Hyunju Ro and Kim, {Dong Il} and Shin, {Yong Hwan} and Kim, {Ji Eun} and Hwang, {Kyu Seok} and Choi, {Jung Hwa} and Minjin Bahn and Lee, {Jeong Ju} and Lee, {Sang H.} and Bae, {Young Ki} and Lee, {Jin Soo} and Choi, {Joong Kook} and Kim, {Nam Soon} and Yeo, {Chang Yeol} and Kim, {Cheol Hee}",

note = "Funding Information: This work was supported by research grants from the National Cancer Center Grant (NCC1210360), the KRIBB Research Initiative Program, and National Research Foundation of Korea (NRF) grants funded by the Korean government Ministry of Science, ICT and Future Planning (MSIP) (2012R1A5A1048236, 2014R1A2A1A11053562, 2015M3A9A8029261). Publisher Copyright: {\textcopyright} 2017 The Author(s).",

year = "2017",

month = dec,

day = "1",

doi = "10.1038/s41598-017-13429-6",

language = "English",

volume = "7",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

number = "1",

}

TY - JOUR

T1 - Ottogi Inhibits Wnt/β-catenin Signaling by Regulating Cell Membrane Trafficking of Frizzled8

AU - Kim, Hyun Taek

AU - Lee, Mi Sun

AU - Jeong, Yun Mi

AU - Ro, Hyunju

AU - Kim, Dong Il

AU - Shin, Yong Hwan

AU - Kim, Ji Eun

AU - Hwang, Kyu Seok

AU - Choi, Jung Hwa

AU - Bahn, Minjin

AU - Lee, Jeong Ju

AU - Lee, Sang H.

AU - Bae, Young Ki

AU - Lee, Jin Soo

AU - Choi, Joong Kook

AU - Kim, Nam Soon

AU - Yeo, Chang Yeol

AU - Kim, Cheol Hee

N1 - Funding Information: This work was supported by research grants from the National Cancer Center Grant (NCC1210360), the KRIBB Research Initiative Program, and National Research Foundation of Korea (NRF) grants funded by the Korean government Ministry of Science, ICT and Future Planning (MSIP) (2012R1A5A1048236, 2014R1A2A1A11053562, 2015M3A9A8029261). Publisher Copyright: © 2017 The Author(s).

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Wnt signaling controls critical developmental processes including tissue/body patterning. Here we report the identification of a novel regulator of Wnt signaling, OTTOGI (OTG), isolated from a large-scale expression screening of human cDNAs in zebrafish embryos. Overexpression of OTG in zebrafish embryos caused dorso-Anteriorized phenotype, inhibited the expression of Wnt target genes, and prevented nuclear accumulation of β-catenin. Conversely, knockdown of zebrafish otg using specific antisense morpholino promoted nuclear accumulation of β-catenin and caused ventralization. However, OTG failed to rescue headless-like phenotype induced by inhibition of GSK-3β activity, suggesting that OTG acts upstream of GSK-3β. OTG bound specifically to Frizzled8 (Fz8) receptor and caused retention of Fz8 in the endoplasmic reticulum possibly by preventing N-linked glycosylation of Fz8. Taken together, our data indicate that OTG functions as a novel negative regulator of Wnt signaling during development by the modulation of cell surface expression of Fz receptor.

AB - Wnt signaling controls critical developmental processes including tissue/body patterning. Here we report the identification of a novel regulator of Wnt signaling, OTTOGI (OTG), isolated from a large-scale expression screening of human cDNAs in zebrafish embryos. Overexpression of OTG in zebrafish embryos caused dorso-Anteriorized phenotype, inhibited the expression of Wnt target genes, and prevented nuclear accumulation of β-catenin. Conversely, knockdown of zebrafish otg using specific antisense morpholino promoted nuclear accumulation of β-catenin and caused ventralization. However, OTG failed to rescue headless-like phenotype induced by inhibition of GSK-3β activity, suggesting that OTG acts upstream of GSK-3β. OTG bound specifically to Frizzled8 (Fz8) receptor and caused retention of Fz8 in the endoplasmic reticulum possibly by preventing N-linked glycosylation of Fz8. Taken together, our data indicate that OTG functions as a novel negative regulator of Wnt signaling during development by the modulation of cell surface expression of Fz receptor.

UR - http://www.scopus.com/inward/record.url?scp=85031779920&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-13429-6

DO - 10.1038/s41598-017-13429-6

M3 - Article

C2 - 29038508

AN - SCOPUS:85031779920

SN - 2045-2322

VL - 7

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 13278

ER -

Ottogi Inhibits Wnt/β-catenin Signaling by Regulating Cell Membrane Trafficking of Frizzled8

Abstract

Access to Document

Fingerprint

Cite this