Ottogi Inhibits Wnt/β-catenin Signaling by Regulating Cell Membrane Trafficking of Frizzled8

Hyun Taek Kim, Mi Sun Lee, Yun Mi Jeong, Hyunju Ro, Dong Il Kim, Yong Hwan Shin, Ji Eun Kim, Kyu Seok Hwang, Jung Hwa Choi, Minjin Bahn, Jeong Ju Lee, Sang H. Lee, Young Ki Bae, Jin Soo Lee, Joong Kook Choi, Nam Soon Kim, Chang Yeol Yeo, Cheol Hee Kim

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Wnt signaling controls critical developmental processes including tissue/body patterning. Here we report the identification of a novel regulator of Wnt signaling, OTTOGI (OTG), isolated from a large-scale expression screening of human cDNAs in zebrafish embryos. Overexpression of OTG in zebrafish embryos caused dorso-Anteriorized phenotype, inhibited the expression of Wnt target genes, and prevented nuclear accumulation of β-catenin. Conversely, knockdown of zebrafish otg using specific antisense morpholino promoted nuclear accumulation of β-catenin and caused ventralization. However, OTG failed to rescue headless-like phenotype induced by inhibition of GSK-3β activity, suggesting that OTG acts upstream of GSK-3β. OTG bound specifically to Frizzled8 (Fz8) receptor and caused retention of Fz8 in the endoplasmic reticulum possibly by preventing N-linked glycosylation of Fz8. Taken together, our data indicate that OTG functions as a novel negative regulator of Wnt signaling during development by the modulation of cell surface expression of Fz receptor.

Original languageEnglish
Article number13278
JournalScientific Reports
Issue number1
StatePublished - 1 Dec 2017

Bibliographical note

Funding Information:
This work was supported by research grants from the National Cancer Center Grant (NCC1210360), the KRIBB Research Initiative Program, and National Research Foundation of Korea (NRF) grants funded by the Korean government Ministry of Science, ICT and Future Planning (MSIP) (2012R1A5A1048236, 2014R1A2A1A11053562, 2015M3A9A8029261).

Publisher Copyright:
© 2017 The Author(s).


Dive into the research topics of 'Ottogi Inhibits Wnt/β-catenin Signaling by Regulating Cell Membrane Trafficking of Frizzled8'. Together they form a unique fingerprint.

Cite this