@inproceedings{764ece97bf5b4e04a7356ce70e167e19,
title = "Opto-chemical disinfection of bacterial pathogens in mature biofilms grown in flexible endoscope",
abstract = "Flexible endoscope reprocessing is an important requirement to minimize the risk of cross-infection between patients due to incomplete disinfection of a bacteria biofilm. The present study introduces a novel opto-chemical treatment to disinfect microbial biofilms (both Gram-positive and Gram-negative bacterial biofilms), commonly found in flexible endoscopes. A low concentration disinfectant combined with infrared and blue light irradiations was applied to disinfect the bacterial biofilms in the endoscope. A basket-integrated optical device was designed to deliver uniform and concentric light onto the channel surface of the endoscope. Colony-forming unit assays were performed to quantify the vial cells while scanning electron microscopy (SEM) illustrated an extracellular matrix (ECM) of the bacterial biofilm. The infrared light irradiation heated the surface of the bacterial biofilm to ~ 65oC. The blue light irradiation induced a relative temperature increase of 30oC on the bacterial biofilm. The results showed that the opto-chemical treatment reduced approximately 7.5-log10 of the bacterial biofilm, which was four times higher than that of a standard disinfectant solution (2% glutaraldehyde). In comparison with the control untreated samples with intact ECMs, the SEM images showed significant damage to the bacterial biofilm under the opto-chemical treatment. The combined treatment induced antimicrobial effects in terms of inhibition of protein synthesis, thermal destruction, and oxidative stress, eradicating the bacterial biofilm more than the standard chemical disinfection. The proposed technique could be an alternative approach to disinfect the microbial biofilms and minimize the risk of secondary infection in endoscopy-related medical facilities.",
keywords = "Bacteria biofilm, Blue light, Disinfection, Flexible endoscope, Glutaraldehyde, Infrared light",
author = "Tran, {Van Nam} and Saravana, {Periaswamy Sivagnanam} and Suhyun Park and Fazlurrahman Khan and Truong, {Van Gia} and Seok Jeong and Lee, {Don Haeng} and Chun, {Byung Soo} and Kim, {Young Mog} and Kang, {Hyun Wook}",
note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2021R1A2C2003733) and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (No. 2021R1A6A1A03039211). Publisher Copyright: {\textcopyright} 2022 SPIE.; 2021 SPIE Advanced Biophotonics Conference, SPIE ABC 2021 ; Conference date: 04-11-2021 Through 06-11-2021",
year = "2021",
doi = "10.1117/12.2626049",
language = "English",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
publisher = "SPIE",
editor = "Euiheon Chung and Ki-Hun Jeong and Chulmin Joo and Woonggyu Jung and Hyun-Wook Kang and Chang-Seok Kim and Chulhong Kim and Pilhan Kim and Hongki Yoo",
booktitle = "SPIE Advanced Biophotonics Conference, SPIE ABC 2021",
}