Optimization of leucrose production by dextransucrase from Streptococcus mutans and its application as an adipogenesis regulator

Daeyeon Lee, Jihye Lee, Moon Gi Hong, Byung Hoo Lee, Young Min Kim, Pahn Shick Chang, Yuri Kim, Sang Ho Yoo

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Leucrose is a sucrose isomer which has an α-1,5-linkage, and slowly hydrolyzed to glucose and fructose by small intestinal α-glucosidases. Leucrose can be produced by an isomerization reaction of dextransucrase on a sucrose substrate. In this study, the recombinant dextransucrase from Streptococcus mutans (SmDS) was applied to optimize the reaction conditions for leucrose production. With a substrate mixture of 0.5 M sucrose + 1.0 M fructose, the greatest yield (ca. 24.5%) of leucrose was obtained by SmDS treatment at 30 °C for 120 h. When preadipocyte 3T3-L1 cells were treated with leucrose, this disaccharide inhibited intracellular lipid accumulation in a dose-dependent manner and significantly suppressed mRNA levels of major adipogenic genes, including CCAT/enhancer-binding protein α (C/EBPα), peroxisome proliferator-activated receptor-γ (PPARγ), fatty acid synthase (FAS), and sterol regulatory element-binding protein-1C (SREBP-1C). Phosphorylation of PI3 kinase/Akt/mTOR was also reduced with leucrose treatment. These results suggest that leucrose has a potential in regulating adipogenesis.

Original languageEnglish
Pages (from-to)238-244
Number of pages7
JournalJournal of Functional Foods
Volume39
DOIs
StatePublished - Dec 2017

Keywords

  • Adipogenesis
  • Dextransucrase
  • Leucrose
  • Streptococcus mutans
  • Sucrose

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