Optimal concentration of human epidermal growth factor (hEGF) for epithelial healing in experimental corneal alkali wounds

M. J. Kim; R. M. Jun; W. K. Kim; H. J. Hann; Y. H. Chong; H. Y. Park; J. H. Chung

doi:10.1076/ceyr.22.4.272.5507

Optimal concentration of human epidermal growth factor (hEGF) for epithelial healing in experimental corneal alkali wounds

M. J. Kim, R. M. Jun, W. K. Kim, H. J. Hann, Y. H. Chong, H. Y. Park, J. H. Chung

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Purpose. By using bothin vivo andin vitro (organ-cultured) systems, the optimal concentrations of hEGF to enhance epithelial healing after alkali wounds were evaluated in the rabbit cornea. Methods. Alkali-injured corneas (φ = 5.5 mm, 1 N NaOH, 60 s) were treated with 0.01, 0.1, 1.0, 10 and 100 ng/ml hEGF for thein vitro study. The healing of epithelium and endothelium was determined at 1, 2, 3, 4, and 6 days after treatment. For thein vivo experiment, the eyes were treated with 2, 5, 10, and 50 mg/ml hEGF 3 times per day. The measurement of epithelial healing rate, transmission electron microscopy and immunohistochemical observation were performed after 7 days treatment. Results. In in vitro tests, hEGF enhanced the epithelial healing rates, showing a maximum enhancement at the concentration of 1.0 ng/ml, and endothelial healing was increased at 100 ng/ml. In in vivo studies, no significant difference was observed in the rates of epithelial healing between control and each hEGF-treated group. Among the tested concentrations, 5 μg/ml hEGF induced the most active proliferation of basal cells and 50 μg/ml hEGF remarkably produced a vascular ingrowth to the central wound area. The thickness of re-surfaced epithelium was increased by hEGF in a concentration-dependent manner. Conclusions. The results of the present study indicate that a low concentration of hEGF may selectively enhance epithelial healing without affecting endothelial healing. The optimal concentration of hEGF for the stimulation of epithelial healing appears to be 5 μg/ml in rabbit corneal alkali wounds.

Original language	English
Pages (from-to)	272-279
Number of pages	8
Journal	Current Eye Research
Volume	22
Issue number	4
DOIs	https://doi.org/10.1076/ceyr.22.4.272.5507
State	Published - 2001

Bibliographical note

Funding Information:
This study was supported by a grant of the Korea Research Foundation made in the Program Year 1997–1998 and Brain Korea 21 Project in 2001. The authors wish to thank DaeWoong Phama. Co. Ltd. for their kind donation of hEGF.

Keywords

Alkali wound
Cornea
Epidermal growth factor (EGF)
Epithelial healing
Rabbit

Access to Document

10.1076/ceyr.22.4.272.5507

Cite this

@article{325e25573f4646e6b87e2a775ccc4f1f,

title = "Optimal concentration of human epidermal growth factor (hEGF) for epithelial healing in experimental corneal alkali wounds",

abstract = "Purpose. By using bothin vivo andin vitro (organ-cultured) systems, the optimal concentrations of hEGF to enhance epithelial healing after alkali wounds were evaluated in the rabbit cornea. Methods. Alkali-injured corneas (φ = 5.5 mm, 1 N NaOH, 60 s) were treated with 0.01, 0.1, 1.0, 10 and 100 ng/ml hEGF for thein vitro study. The healing of epithelium and endothelium was determined at 1, 2, 3, 4, and 6 days after treatment. For thein vivo experiment, the eyes were treated with 2, 5, 10, and 50 mg/ml hEGF 3 times per day. The measurement of epithelial healing rate, transmission electron microscopy and immunohistochemical observation were performed after 7 days treatment. Results. In in vitro tests, hEGF enhanced the epithelial healing rates, showing a maximum enhancement at the concentration of 1.0 ng/ml, and endothelial healing was increased at 100 ng/ml. In in vivo studies, no significant difference was observed in the rates of epithelial healing between control and each hEGF-treated group. Among the tested concentrations, 5 μg/ml hEGF induced the most active proliferation of basal cells and 50 μg/ml hEGF remarkably produced a vascular ingrowth to the central wound area. The thickness of re-surfaced epithelium was increased by hEGF in a concentration-dependent manner. Conclusions. The results of the present study indicate that a low concentration of hEGF may selectively enhance epithelial healing without affecting endothelial healing. The optimal concentration of hEGF for the stimulation of epithelial healing appears to be 5 μg/ml in rabbit corneal alkali wounds.",

keywords = "Alkali wound, Cornea, Epidermal growth factor (EGF), Epithelial healing, Rabbit",

author = "Kim, {M. J.} and Jun, {R. M.} and Kim, {W. K.} and Hann, {H. J.} and Chong, {Y. H.} and Park, {H. Y.} and Chung, {J. H.}",

note = "Funding Information: This study was supported by a grant of the Korea Research Foundation made in the Program Year 1997–1998 and Brain Korea 21 Project in 2001. The authors wish to thank DaeWoong Phama. Co. Ltd. for their kind donation of hEGF.",

year = "2001",

doi = "10.1076/ceyr.22.4.272.5507",

language = "English",

volume = "22",

pages = "272--279",

journal = "Current Eye Research",

issn = "0271-3683",

publisher = "Taylor and Francis Ltd.",

number = "4",

}

TY - JOUR

T1 - Optimal concentration of human epidermal growth factor (hEGF) for epithelial healing in experimental corneal alkali wounds

AU - Kim, M. J.

AU - Jun, R. M.

AU - Kim, W. K.

AU - Hann, H. J.

AU - Chong, Y. H.

AU - Park, H. Y.

AU - Chung, J. H.

N1 - Funding Information: This study was supported by a grant of the Korea Research Foundation made in the Program Year 1997–1998 and Brain Korea 21 Project in 2001. The authors wish to thank DaeWoong Phama. Co. Ltd. for their kind donation of hEGF.

PY - 2001

Y1 - 2001

N2 - Purpose. By using bothin vivo andin vitro (organ-cultured) systems, the optimal concentrations of hEGF to enhance epithelial healing after alkali wounds were evaluated in the rabbit cornea. Methods. Alkali-injured corneas (φ = 5.5 mm, 1 N NaOH, 60 s) were treated with 0.01, 0.1, 1.0, 10 and 100 ng/ml hEGF for thein vitro study. The healing of epithelium and endothelium was determined at 1, 2, 3, 4, and 6 days after treatment. For thein vivo experiment, the eyes were treated with 2, 5, 10, and 50 mg/ml hEGF 3 times per day. The measurement of epithelial healing rate, transmission electron microscopy and immunohistochemical observation were performed after 7 days treatment. Results. In in vitro tests, hEGF enhanced the epithelial healing rates, showing a maximum enhancement at the concentration of 1.0 ng/ml, and endothelial healing was increased at 100 ng/ml. In in vivo studies, no significant difference was observed in the rates of epithelial healing between control and each hEGF-treated group. Among the tested concentrations, 5 μg/ml hEGF induced the most active proliferation of basal cells and 50 μg/ml hEGF remarkably produced a vascular ingrowth to the central wound area. The thickness of re-surfaced epithelium was increased by hEGF in a concentration-dependent manner. Conclusions. The results of the present study indicate that a low concentration of hEGF may selectively enhance epithelial healing without affecting endothelial healing. The optimal concentration of hEGF for the stimulation of epithelial healing appears to be 5 μg/ml in rabbit corneal alkali wounds.

AB - Purpose. By using bothin vivo andin vitro (organ-cultured) systems, the optimal concentrations of hEGF to enhance epithelial healing after alkali wounds were evaluated in the rabbit cornea. Methods. Alkali-injured corneas (φ = 5.5 mm, 1 N NaOH, 60 s) were treated with 0.01, 0.1, 1.0, 10 and 100 ng/ml hEGF for thein vitro study. The healing of epithelium and endothelium was determined at 1, 2, 3, 4, and 6 days after treatment. For thein vivo experiment, the eyes were treated with 2, 5, 10, and 50 mg/ml hEGF 3 times per day. The measurement of epithelial healing rate, transmission electron microscopy and immunohistochemical observation were performed after 7 days treatment. Results. In in vitro tests, hEGF enhanced the epithelial healing rates, showing a maximum enhancement at the concentration of 1.0 ng/ml, and endothelial healing was increased at 100 ng/ml. In in vivo studies, no significant difference was observed in the rates of epithelial healing between control and each hEGF-treated group. Among the tested concentrations, 5 μg/ml hEGF induced the most active proliferation of basal cells and 50 μg/ml hEGF remarkably produced a vascular ingrowth to the central wound area. The thickness of re-surfaced epithelium was increased by hEGF in a concentration-dependent manner. Conclusions. The results of the present study indicate that a low concentration of hEGF may selectively enhance epithelial healing without affecting endothelial healing. The optimal concentration of hEGF for the stimulation of epithelial healing appears to be 5 μg/ml in rabbit corneal alkali wounds.

KW - Alkali wound

KW - Cornea

KW - Epidermal growth factor (EGF)

KW - Epithelial healing

KW - Rabbit

UR - http://www.scopus.com/inward/record.url?scp=0034821687&partnerID=8YFLogxK

U2 - 10.1076/ceyr.22.4.272.5507

DO - 10.1076/ceyr.22.4.272.5507

M3 - Article

C2 - 11462166

AN - SCOPUS:0034821687

SN - 0271-3683

VL - 22

SP - 272

EP - 279

JO - Current Eye Research

JF - Current Eye Research

IS - 4

ER -

Optimal concentration of human epidermal growth factor (hEGF) for epithelial healing in experimental corneal alkali wounds

Abstract

Bibliographical note

Keywords

Access to Document

Fingerprint

Cite this