TY - JOUR
T1 - Optimal concentration of human epidermal growth factor (hEGF) for epithelial healing in experimental corneal alkali wounds
AU - Kim, M. J.
AU - Jun, R. M.
AU - Kim, W. K.
AU - Hann, H. J.
AU - Chong, Y. H.
AU - Park, H. Y.
AU - Chung, J. H.
N1 - Funding Information:
This study was supported by a grant of the Korea Research Foundation made in the Program Year 1997–1998 and Brain Korea 21 Project in 2001. The authors wish to thank DaeWoong Phama. Co. Ltd. for their kind donation of hEGF.
PY - 2001
Y1 - 2001
N2 - Purpose. By using bothin vivo andin vitro (organ-cultured) systems, the optimal concentrations of hEGF to enhance epithelial healing after alkali wounds were evaluated in the rabbit cornea. Methods. Alkali-injured corneas (φ = 5.5 mm, 1 N NaOH, 60 s) were treated with 0.01, 0.1, 1.0, 10 and 100 ng/ml hEGF for thein vitro study. The healing of epithelium and endothelium was determined at 1, 2, 3, 4, and 6 days after treatment. For thein vivo experiment, the eyes were treated with 2, 5, 10, and 50 mg/ml hEGF 3 times per day. The measurement of epithelial healing rate, transmission electron microscopy and immunohistochemical observation were performed after 7 days treatment. Results. In in vitro tests, hEGF enhanced the epithelial healing rates, showing a maximum enhancement at the concentration of 1.0 ng/ml, and endothelial healing was increased at 100 ng/ml. In in vivo studies, no significant difference was observed in the rates of epithelial healing between control and each hEGF-treated group. Among the tested concentrations, 5 μg/ml hEGF induced the most active proliferation of basal cells and 50 μg/ml hEGF remarkably produced a vascular ingrowth to the central wound area. The thickness of re-surfaced epithelium was increased by hEGF in a concentration-dependent manner. Conclusions. The results of the present study indicate that a low concentration of hEGF may selectively enhance epithelial healing without affecting endothelial healing. The optimal concentration of hEGF for the stimulation of epithelial healing appears to be 5 μg/ml in rabbit corneal alkali wounds.
AB - Purpose. By using bothin vivo andin vitro (organ-cultured) systems, the optimal concentrations of hEGF to enhance epithelial healing after alkali wounds were evaluated in the rabbit cornea. Methods. Alkali-injured corneas (φ = 5.5 mm, 1 N NaOH, 60 s) were treated with 0.01, 0.1, 1.0, 10 and 100 ng/ml hEGF for thein vitro study. The healing of epithelium and endothelium was determined at 1, 2, 3, 4, and 6 days after treatment. For thein vivo experiment, the eyes were treated with 2, 5, 10, and 50 mg/ml hEGF 3 times per day. The measurement of epithelial healing rate, transmission electron microscopy and immunohistochemical observation were performed after 7 days treatment. Results. In in vitro tests, hEGF enhanced the epithelial healing rates, showing a maximum enhancement at the concentration of 1.0 ng/ml, and endothelial healing was increased at 100 ng/ml. In in vivo studies, no significant difference was observed in the rates of epithelial healing between control and each hEGF-treated group. Among the tested concentrations, 5 μg/ml hEGF induced the most active proliferation of basal cells and 50 μg/ml hEGF remarkably produced a vascular ingrowth to the central wound area. The thickness of re-surfaced epithelium was increased by hEGF in a concentration-dependent manner. Conclusions. The results of the present study indicate that a low concentration of hEGF may selectively enhance epithelial healing without affecting endothelial healing. The optimal concentration of hEGF for the stimulation of epithelial healing appears to be 5 μg/ml in rabbit corneal alkali wounds.
KW - Alkali wound
KW - Cornea
KW - Epidermal growth factor (EGF)
KW - Epithelial healing
KW - Rabbit
UR - http://www.scopus.com/inward/record.url?scp=0034821687&partnerID=8YFLogxK
U2 - 10.1076/ceyr.22.4.272.5507
DO - 10.1076/ceyr.22.4.272.5507
M3 - Article
C2 - 11462166
AN - SCOPUS:0034821687
VL - 22
SP - 272
EP - 279
JO - Current Eye Research
JF - Current Eye Research
SN - 0271-3683
IS - 4
ER -