Optical methods in studies of olfactory system

Sang Hun Lee; Seung Min Park; Luke P. Lee

doi:10.1007/978-94-017-8613-3_11

Optical methods in studies of olfactory system

Sang Hun Lee, Seung Min Park, Luke P. Lee

Department of Chemistry & Nanoscience

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

The olfactory receptor (OR) comprises the largest multi-gene G protein-coupled receptor (GPCR) family by playing a critical role in recognizing thousands of odorant molecules. Odorant-OR pairs have been characterized using various functional assays, and have provided an understanding of molecular basis in olfaction as well as characterizing specificity between agonist and antagonist. This chapter introduces the most commonly employed, labeled or label-free optical techniques employed to identify the odorant-OR pairs on a cellular and molecular level, and reviews recent developments in odorant binding assays to ORs with optical methods such as Ca²⁺ imaging, reporter-gene technology, surface plasmon resonance (SPR) and so on. For OR and GPCR study, a set of optical technologies including-but not limited to-Raman spectroscopy, photonic crystal, and total internal reflection (TIR) are also discussed in an analytical science point of view.

Original language	English
Title of host publication	Bioelectronic Nose
Subtitle of host publication	Integration of Biotechnology and Nanotechnology
Publisher	Springer Netherlands
Pages	191-220
Number of pages	30
Volume	9789401786133
ISBN (Electronic)	9789401786133
ISBN (Print)	9401786127, 9789401786126
DOIs	https://doi.org/10.1007/978-94-017-8613-3_11
State	Published - 1 Dec 2014

Bibliographical note

Publisher Copyright:
© 2014 Springer Science+Business Media Dordrecht.

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10.1007/978-94-017-8613-3_11

Cite this

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Optical methods in studies of olfactory system

Abstract

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