@inproceedings{95738e036f934f5995b5e587c56c4103,
title = "One-step digital plasma separation for molecular diagnostics",
abstract = "Current blood-based quantitative nucleic acid (NA) detection requires many sample preparation steps. We have developed the digital plasma separation platform, which combines autonomous parallel plasma separation and sample compartmentalization for digital NA amplification in one-step. For NA detection we utilized isothermal Recombinase Polymerase Amplification (RPA). Simple fluorescence endpoint counts of positive wells can be done by a standard microscope, making it possible to be an alternative low cost solution compared to RT-PCR. We demonstrated digital amplification from spiked samples and the detection of Methicillin-Resistant Staphylococcus Aureus (MRSA) DNA directly from blood samples in 30 min with DPS.",
keywords = "Digital amplification, Isothermal amplification, MRSA, Plasma separation, Recombinase Polymerase Amplification, RT-PCR, Sample preparation",
author = "Yeh, {Erh Chia} and Lee, {Luke P.}",
year = "2013",
language = "English",
isbn = "9781632666246",
series = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
publisher = "Chemical and Biological Microsystems Society",
pages = "1323--1325",
booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
note = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 ; Conference date: 27-10-2013 Through 31-10-2013",
}