One-step digital plasma separation for molecular diagnostics

Erh Chia Yeh, Luke P. Lee

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

7 Scopus citations

Abstract

Current blood-based quantitative nucleic acid (NA) detection requires many sample preparation steps. We have developed the digital plasma separation platform, which combines autonomous parallel plasma separation and sample compartmentalization for digital NA amplification in one-step. For NA detection we utilized isothermal Recombinase Polymerase Amplification (RPA). Simple fluorescence endpoint counts of positive wells can be done by a standard microscope, making it possible to be an alternative low cost solution compared to RT-PCR. We demonstrated digital amplification from spiked samples and the detection of Methicillin-Resistant Staphylococcus Aureus (MRSA) DNA directly from blood samples in 30 min with DPS.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1323-1325
Number of pages3
ISBN (Print)9781632666246
StatePublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 27 Oct 201331 Oct 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume2

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Country/TerritoryGermany
CityFreiburg
Period27/10/1331/10/13

Keywords

  • Digital amplification
  • Isothermal amplification
  • MRSA
  • Plasma separation
  • Recombinase Polymerase Amplification
  • RT-PCR
  • Sample preparation

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