One-Photon and Two-Photon Sensing of Biothiols Using a Bis-Pyrene-Cu(II) Ensemble and Its Application to Image GSH in the Cells and Tissues

Ying Hu, Cheol Ho Heo, Gyoungmi Kim, Eun Jin Jun, Jun Yin, Hwan Myung Kim, Juyoung Yoon

Research output: Contribution to journalArticlepeer-review

93 Scopus citations

Abstract

Glutathione (GSH), cysteine (Cys), and homocysteine (Hcy) are three major biothiols, which play key roles in various biological systems. Accordingly, the development of imaging probes has been actively studied. We report a new pyrene derivative 1, which showed large fluorescence quenching with Cu2+ at pH 7.4. The ensemble 1-Cu2+ was applied to detect biothiols. Among the various amino acids, GSH, Cys, and Hcy induced distinct turn-on fluorescence changes. The 1-Cu2+ ensemble was further applied for GSH detection in living cells. (Figure Presented).

Original languageEnglish
Pages (from-to)3308-3313
Number of pages6
JournalAnalytical Chemistry
Volume87
Issue number6
DOIs
StatePublished - 17 Mar 2015

Bibliographical note

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© 2015 American Chemical Society.

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