Occurrence of patulin in various fruit juices from south Korea: An exposure assessment

Mi Sook Cho, Kyeongyeol Kim, Eunkyoung Seo, Neema Kassim, Adelard Barthalomew Mtenga, Won Bo Shim, Soo Hyung Lee, Duck Hwa Chung

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28 Scopus citations


To determine patulin in various fruit juices, high performance liquid chromatography (HPLC) method was optimized and validated. For validation of HPLC method, a linearity, accuracy, precision, detection limit, and quantification limit were determined. Linearity (R2= 0.99995), accuracy (96.1-115.7%), precision (3.31-9.52), detection limit (6 ng/mL), and quantification limit (8 ng/ mL) were in agreement with performance criteria for patulin as set by the European Commission hence proved that HPLC can be used to detect patulin in fruit juices. After validation, the method was applied to estimate the prevalence of patulin in fruit juices (apple, grape, and orange juices). Nine samples (12.5%, 3 apple, 2 orange, and 4 grape juices) of 72 samples were positive for patulin in the range 2.8 to 30.9 ng/mL. According to the monitoring results, daily intake was estimated to be 0.17 ng/kg BW/ day which was lower than the provisional maximum tolerable daily intake (0.4 μg/kg) established by Joint Expert Committee on Food Additives. These results indicate that the detection method coincides with the performance criteria and is appropriate for analysis of patulin, and continuous monitoring of patulin in various fruit juices from Korea is necessary.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalFood Science and Biotechnology
Issue number1
StatePublished - Feb 2010

Bibliographical note

Funding Information:
Acknowledgments The authors would like to thank financial support from the National Core Research Center and Rural Development Administration. Kyeongyeol Kim and Neema Kassim were supported by the Brain Korea 21 Program (BK21) from the Korean Ministry of Education.


  • Exposure assessment
  • Fruit juice
  • Mycotoxin
  • Patulin
  • Validation


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