Observing hydrogen sulfide in the endoplasmic reticulum of cancer cells and zebrafish by using an activity-based fluorescent probe

A crucial endogenous signaling chemical, hydrogen sulfide, is involved in many physiological actions. In this work, we created the fluorescent probe ER-Nap-NBD using a naphthalimide fluorophore as the signal reporter, a 7-nitro-1,2,3-benzoxadiazole amine as the responsive moiety, and a sulfonamide part for endoplasmic reticulum targeting. ER-Nap-NBD could be detected the H₂S levels in solution and in living systems (cells and zebrafish).