Observation of self-assembled fluorescent beads by scanning near-field optical microscopy and atomic force microscopy

Y. J. Oh; W. Jo; Min Gon Kim; Hyun Kyu Park; Bong Hyun Chung

doi:10.1016/j.ultramic.2005.12.014

Observation of self-assembled fluorescent beads by scanning near-field optical microscopy and atomic force microscopy

Y. J. Oh, W. Jo, Min Gon Kim, Hyun Kyu Park, Bong Hyun Chung

Department of Physics

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Optical response and topography of fluorescent latex beads both on flat self-assembled monolayer and on a micron-patterned surface with poly(dimethylsiloxane) are studied. Scanning near-field optical microscopy and atomic force microscopy were utilized together for detecting fluorescence and imaging topography of the patterned latex beads, respectively. As a result, the micro-patterned latex beads where a specific chemical binding occurred show a strong signal, whereas no signals are observed in the case of nonspecific binding. With fluorescein isothiocyanate (FITC), it is convenient to measure fluorescence signal from the patterned beads allowing us to monitor the small balls of fluorescent latex.

Original language	English
Pages (from-to)	775-778
Number of pages	4
Journal	Ultramicroscopy
Volume	106
Issue number	8-9
DOIs	https://doi.org/10.1016/j.ultramic.2005.12.014
State	Published - Jun 2006

Bibliographical note

Funding Information:
This research was supported by a Grant (code #: 05K1501-02520) from ‘Center for Nanostructured Materials Technology’ under ‘21st Century Frontier R&D Programs’ of the Ministry of Science and Technology, Korea.

Keywords

Beads
Fluorescence detection
SNOM
Self-assembled monolayers

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10.1016/j.ultramic.2005.12.014

Cite this

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title = "Observation of self-assembled fluorescent beads by scanning near-field optical microscopy and atomic force microscopy",

abstract = "Optical response and topography of fluorescent latex beads both on flat self-assembled monolayer and on a micron-patterned surface with poly(dimethylsiloxane) are studied. Scanning near-field optical microscopy and atomic force microscopy were utilized together for detecting fluorescence and imaging topography of the patterned latex beads, respectively. As a result, the micro-patterned latex beads where a specific chemical binding occurred show a strong signal, whereas no signals are observed in the case of nonspecific binding. With fluorescein isothiocyanate (FITC), it is convenient to measure fluorescence signal from the patterned beads allowing us to monitor the small balls of fluorescent latex.",

keywords = "Beads, Fluorescence detection, SNOM, Self-assembled monolayers",

author = "Oh, {Y. J.} and W. Jo and Kim, {Min Gon} and {Kyu Park}, Hyun and {Hyun Chung}, Bong",

note = "Funding Information: This research was supported by a Grant (code #: 05K1501-02520) from {\textquoteleft}Center for Nanostructured Materials Technology{\textquoteright} under {\textquoteleft}21st Century Frontier R&D Programs{\textquoteright} of the Ministry of Science and Technology, Korea.",

year = "2006",

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doi = "10.1016/j.ultramic.2005.12.014",

language = "English",

volume = "106",

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journal = "Ultramicroscopy",

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AU - Oh, Y. J.

AU - Jo, W.

AU - Kim, Min Gon

AU - Kyu Park, Hyun

AU - Hyun Chung, Bong

N1 - Funding Information: This research was supported by a Grant (code #: 05K1501-02520) from ‘Center for Nanostructured Materials Technology’ under ‘21st Century Frontier R&D Programs’ of the Ministry of Science and Technology, Korea.

PY - 2006/6

Y1 - 2006/6

N2 - Optical response and topography of fluorescent latex beads both on flat self-assembled monolayer and on a micron-patterned surface with poly(dimethylsiloxane) are studied. Scanning near-field optical microscopy and atomic force microscopy were utilized together for detecting fluorescence and imaging topography of the patterned latex beads, respectively. As a result, the micro-patterned latex beads where a specific chemical binding occurred show a strong signal, whereas no signals are observed in the case of nonspecific binding. With fluorescein isothiocyanate (FITC), it is convenient to measure fluorescence signal from the patterned beads allowing us to monitor the small balls of fluorescent latex.

AB - Optical response and topography of fluorescent latex beads both on flat self-assembled monolayer and on a micron-patterned surface with poly(dimethylsiloxane) are studied. Scanning near-field optical microscopy and atomic force microscopy were utilized together for detecting fluorescence and imaging topography of the patterned latex beads, respectively. As a result, the micro-patterned latex beads where a specific chemical binding occurred show a strong signal, whereas no signals are observed in the case of nonspecific binding. With fluorescein isothiocyanate (FITC), it is convenient to measure fluorescence signal from the patterned beads allowing us to monitor the small balls of fluorescent latex.

KW - Beads

KW - Fluorescence detection

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KW - Self-assembled monolayers

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ER -

Observation of self-assembled fluorescent beads by scanning near-field optical microscopy and atomic force microscopy

Abstract

Bibliographical note

Keywords

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