Observation of self-assembled fluorescent beads by scanning near-field optical microscopy and atomic force microscopy

Y. J. Oh, W. Jo, Min Gon Kim, Hyun Kyu Park, Bong Hyun Chung

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Optical response and topography of fluorescent latex beads both on flat self-assembled monolayer and on a micron-patterned surface with poly(dimethylsiloxane) are studied. Scanning near-field optical microscopy and atomic force microscopy were utilized together for detecting fluorescence and imaging topography of the patterned latex beads, respectively. As a result, the micro-patterned latex beads where a specific chemical binding occurred show a strong signal, whereas no signals are observed in the case of nonspecific binding. With fluorescein isothiocyanate (FITC), it is convenient to measure fluorescence signal from the patterned beads allowing us to monitor the small balls of fluorescent latex.

Original languageEnglish
Pages (from-to)775-778
Number of pages4
JournalUltramicroscopy
Volume106
Issue number8-9
DOIs
StatePublished - Jun 2006

Bibliographical note

Funding Information:
This research was supported by a Grant (code #: 05K1501-02520) from ‘Center for Nanostructured Materials Technology’ under ‘21st Century Frontier R&D Programs’ of the Ministry of Science and Technology, Korea.

Keywords

  • Beads
  • Fluorescence detection
  • Self-assembled monolayers
  • SNOM

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