Nucleotide and deduced amino acid sequences of rat myosin binding protein H (MyBP-H)

Jaehoon Jung, Jihyun Oh, Kyunglim Lee

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The complete nucleotide sequence of the cDNA clone encoding rat skeletal muscle myosin-binding protein H (MyBP-H) was determined and amino acid sequence was deduced from the nucleotide sequence (GenBank accession number AF077338). The full-length cDNA of 1782 base pairs(bp) contains a single open reading frame of 1454 bp encoding a rat MyBP-H protein of the predicted molecular mass 52.7kDa and includes the common consensus 'CA_ _TG' protein binding motif. The cDNA sequence of rat MyBP-H show 92%, 84% and 41% homology with those of mouse, human and chicken, respectively. The protein contains tandem internal motifs array (-FN III-Ig C2-FN III- Ig C2-) in the C-terminal region which resembles to the immunoglobulin superfamily C2 and fibronectin type III motifs. The amino acid sequence of the C-terminal Ig C2 was highly conserved among MyBPs family and other thick filament binding proteins, suggesting that the C-terminal Ig C2 might play an important role in its function. All proteins belonging to MyBP-H member contains 'RKPS' sequence which is assumed to be cAMP- and cGMP-dependent protein kinase A phosphorylation site. Computer analysis of the primary sequence of rat MyBP-H predicted 11 protein kinase C (PKC) phosphorylation site, 7 casein kinase II (CK2) phosphorylation site and 4 N-myristoylation site.

Original languageEnglish
Pages (from-to)712-717
Number of pages6
JournalArchives of Pharmacal Research
Issue number6
StatePublished - Dec 1998

Bibliographical note

Funding Information:
We thank Dr. II Yeong Park for helping with the usage of PDB. This study was supported by the Most through the Women's University Research Fund.


  • Fibronectin type III
  • Immunoglobulin superfamily C2
  • Myosin binding protein H (MyBP-H)


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