Novel strategy for lipase-catalyzed synthesis of biodiesel using blended alcohol as an acyl acceptor

Tingting Zhao, Da Som No, Yangha Kim, Young Soon Kim, In Hwan Kim

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19 Scopus citations


As a novel strategy, blended alcohols consisting of methanol and ethanol were used as acyl acceptors for biodiesel synthesis from soybean oil by lipase-catalyzed transesterification. Based on enzyme screening, Novozym 435 from Candida antarctica was selected for the reaction. The effects of the molar proportion of methanol in the blended alcohol, temperature, and enzyme loading were investigated for optimization of the reaction. In addition, the relative consumption rates of methanol and ethanol during the transesterification were studied. Among six proportions tested, 0 (100 mol% ethanol), 20, 40, and 60 mol% methanol in the blended alcohols exhibited high yields of biodiesel. For the optimum temperature, 30 °C was selected. The highest yield of biodiesel, over 95 wt%, was obtained at an enzyme loading of 5-10 wt% loading. In the lipase-catalyzed transesterification, the reactivity of methanol was significantly higher than that of ethanol.

Original languageEnglish
Pages (from-to)17-22
Number of pages6
JournalJournal of Molecular Catalysis B: Enzymatic
StatePublished - Sep 2014

Bibliographical note

Funding Information:
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science, and Technology ( 2013R1A1A2006050 ).


  • Biodiesel
  • Blended alcohol
  • Ethanol
  • Lipase
  • Methanol


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