We report isolation of novel splice variants of chicken Neuronal Per-. Arnt-. Sim domain protein 3 ( cNPAS3) gene distinct from the previously predicted cNPAS3 at the 5' end. Newly identified cNPAS3 splice variants feature N-terminus coding sequences with high degrees of homology to human NPAS3 ( hNAPS3). We also show that the alternative splicing pattern of NPAS3 is conserved between chicken and human. RNA in situ hybridization indicated that the expression of cNPAS3 in the developing central nervous system (CNS) is limited to the ventricular zone and only partially overlaps with that of chicken Reelin ( cReelin), the only known regulatory target gene of NPAS3 in the adult brain. Overexpression of cNPAS3 by in ovo electroporation had little effect on the expression of Sox2, a marker for neural precursors, or of Isl1/2, a marker for early differentiating motor neurons. Taken together with the little effect of cNPAS3 overexpression on cReelin, it is noted that the function of NPAS3 in the developing CNS remains to be determined. Still, identification of proper cDNA sequences for cNPAS3 should represent a solid beginning of the understanding process.
Bibliographical noteFunding Information:
This research was supported by a grant (2010K000803) from Brain Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science, ICT & Future Planning, Republic of Korea and by the “Systems Biology Infrastructure Establishment Grant” provided by Gwangju Institute of Science and Technology in 2008 through the Ewha Research Center for Systems Biology (ERCSB).
- Alternative splice variants