Nonanimal Euglena gracilis-Derived Extracellular Vesicles Enhance Skin-Regenerative Wound Healing

Yuri Ko, Hwira Baek, Jee Hyun Hwang, Youngseok Kim, Kyung Min Lim, Junoh Kim, Jin Woong Kim

Research output: Contribution to journalArticlepeer-review


This study proposes using microalgae-containing carbohydrate bioactives, an Euglena gracilis-derived extracellular microvesicle (EMVEG) system, for enhanced skin regeneration. The critical deformation ratio, 1.67, during cell extrusion enables the authors to tune the particle size of the EMVEG at ≈1 µm, thus satisfying the encapsulation yield of β-1,3-glucan and the cellular delivery performance. In vitro 5-bromo-2'-deoxyuridine and cell scratch assays reveal that the EMVEG promotes the proliferation and migration of skin cells, thereby increasing both collagen synthesis and the expressions of proliferation-associated proteins. An ex vivo wound-healing test using both artificial and porcine skin reveals that similar to that seen using β-1,3-glucan, the EMVEG can substantially increase the cell population, expressing the proliferation-related protein, termed proliferating cell nuclear antigen. These results demonstrate that the EMVEG system shows considerable potential in the field of skin regeneration. This technique is expected to design new types of extracellular vesicles that are applicable for skin regeneration in the pharmaceutical and cosmetic industries.

Original languageEnglish
Article number2202255
JournalAdvanced Materials Interfaces
Issue number4
StatePublished - 3 Feb 2023

Bibliographical note

Funding Information:
Y.K. and H.B. contributed equally to this work. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (grant Nos. NRF‐2021R1A4A1032782 and NRF‐2021R1A4A5032463) to J.W.K, and (grant No. NRF‐2021R1A2C2013347) to K.M.L.

Publisher Copyright:
© 2023 The Authors. Advanced Materials Interfaces published by Wiley-VCH GmbH.


  • Euglena gracilis
  • extracellular microvesicle
  • skin regeneration
  • β-glucan


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