TY - JOUR
T1 - Nocardiopsis oceanisediminis sp. nov., a Marine-Derived Actinobacterium Isolated from Sediment
AU - Kim, Mirae
AU - Lim, Yeonjung
AU - Lee, Jayho
AU - Nam, Sang Jip
AU - Oh, Dong Chan
AU - Kang, Ilnam
AU - Cho, Jang Cheon
N1 - Publisher Copyright:
© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2025.
PY - 2025/10
Y1 - 2025/10
N2 - Strain CNT-189Τ, previously isolated from marine sediment and identified as an androsamide producer, was taxonomically characterized in this study. Strain CNT-189Τ is a Gram-stain-positive, non-motile, pale yellow-pigmented, aerobic actinomycete. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CNT-189Τ belonged to the genus Nocardiopsis and was most closely related to Nocardiopsis potens DSM 45234Τ (99.6% similarity). Whole-genome sequencing revealed a DNA G + C content of 74.1% and genome size of 7.12 Mbp. Average nucleotide identity (92.8–95.1%) and digital DNA-DNA hybridization values (45.9–55.4%) between strain CNT-189Τ and related species were below the species delineation thresholds, supporting its classification as a novel species. The major respiratory quinone was menaquinone-10 (MK-10) and the predominant cellular fatty acids were C18:1ω9c (25.0%), C16:0 (13.5%), and anteiso-C15:0 (12.7%). Major polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylinositol mannoside, and monomannosyl diglyceride. Whole-cell hydrolysates contained meso-diaminopimelic acid, glycine, and no diagnostic sugars. Based on phylogenetic and phenotypic evidence, strain CNT-189T is considered to represent a novel species of the genus Nocardiopsis, for which the name Nocardiopsis oceanisediminis sp. nov. is proposed. The type strain is CNT-189Τ (= KACC 22136Τ = NBRC 114760Τ).
AB - Strain CNT-189Τ, previously isolated from marine sediment and identified as an androsamide producer, was taxonomically characterized in this study. Strain CNT-189Τ is a Gram-stain-positive, non-motile, pale yellow-pigmented, aerobic actinomycete. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CNT-189Τ belonged to the genus Nocardiopsis and was most closely related to Nocardiopsis potens DSM 45234Τ (99.6% similarity). Whole-genome sequencing revealed a DNA G + C content of 74.1% and genome size of 7.12 Mbp. Average nucleotide identity (92.8–95.1%) and digital DNA-DNA hybridization values (45.9–55.4%) between strain CNT-189Τ and related species were below the species delineation thresholds, supporting its classification as a novel species. The major respiratory quinone was menaquinone-10 (MK-10) and the predominant cellular fatty acids were C18:1ω9c (25.0%), C16:0 (13.5%), and anteiso-C15:0 (12.7%). Major polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylinositol mannoside, and monomannosyl diglyceride. Whole-cell hydrolysates contained meso-diaminopimelic acid, glycine, and no diagnostic sugars. Based on phylogenetic and phenotypic evidence, strain CNT-189T is considered to represent a novel species of the genus Nocardiopsis, for which the name Nocardiopsis oceanisediminis sp. nov. is proposed. The type strain is CNT-189Τ (= KACC 22136Τ = NBRC 114760Τ).
UR - https://www.scopus.com/pages/publications/105013646367
U2 - 10.1007/s00284-025-04444-2
DO - 10.1007/s00284-025-04444-2
M3 - Article
C2 - 40813730
AN - SCOPUS:105013646367
SN - 0343-8651
VL - 82
JO - Current Microbiology
JF - Current Microbiology
IS - 10
M1 - 453
ER -
